Foot-and-Mouth Disease Virus Capsid Protein VP1 Interacts with Host Ribosomal Protein SA To Maintain Activation of the MAPK Signal Pathway and Promote Virus Replication.

Abstract

Foot-and-mouth disease virus (FMDV) is the causative agent of foot-and-mouth disease, a highly contagious, economically important viral disease. The structural protein VP1 plays significant roles during FMDV infection. Here, we identified that VP1 interacted with host ribosomal protein SA (RPSA). RPSA is a viral receptor for dengue virus and classical swine fever virus infections. However, the incubation of susceptible cells using the anti-RPSA antibodies did not block the infection of FMDV. Overexpression of porcine RPSA in the insusceptible cells could not trigger FMDV infection, suggesting that RPSA was not responsible for FMDV entry and infection. On the contrary, we found that overexpression of RPSA suppressed FMDV replication, and knockdown of RPSA enhanced FMDV replication. We further determined that FMDV infection activated the mitogen-activated protein kinase (MAPK) pathway and demonstrated that MAPK pathway activation was critically important for FMDV replication. RPSA negatively regulated MAPK pathway activation during FMDV infection and displayed an antiviral function. FMDV VP1 interacted with RPSA to abrogate the RPSA-mediated suppressive role in MAPK pathway activation. Together, our study indicated that MAPK pathway activation was required for FMDV replication and that host RPSA played a negatively regulatory role on MAPK pathway activation to suppress FMDV replication. FMDV VP1 bound to RPSA to promote viral replication by repressing RPSA-mediated function and maintaining the activation of MAPK signal pathway.IMPORTANCE Identification of virus-cell interactions is essential for making strategies to limit virus replication and refine the models of virus replication. This study demonstrated that FMDV utilized the MAPK pathway for viral replication. The host RPSA protein inhibited FMDV replication by suppressing the activation of the MAPK pathway during FMDV infection. FMDV VP1 bound to RPSA to repress the RPSA-mediated regulatory effect on MAPK pathway activation. This study revealed an important implication of the MAPK pathway for FMDV infection and identified a novel mechanism by which FMDV VP1 has evolved to interact with RPSA and maintain the activation of the MAPK pathway, elucidating new information regarding the signal reprogramming of host cells by FMDV.

• Authors: Zixiang Zhu, Weiwei Li, Xiangle Zhang, Congcong Wang, Lili Gao, Fan Yang, Weijun Cao, Kangli Li, Hong Tian, Xiangtao Liu, Keshan Zhang, Haixue Zheng
• Journal: Journal of virology (J Virol) Vol. 94 Issue 3 (01 17 2020) ISSN: 1098-5514 [Electronic] United States
• PMID: 31694957 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Zhu et al.

Chemical References

• Antiviral Agents
• Capsid Proteins
• RPSA protein, human
• Receptors, Laminin
• Ribosomal Proteins
• VP1 protein, Foot-and-mouth disease virus
• Viral Proteins
• Mitogen-Activated Protein Kinases

Topics

• Animals
• Antiviral Agents (pharmacology)
• Capsid Proteins (metabolism)
• Cell Line
• Foot-and-Mouth Disease (virology)
• Foot-and-Mouth Disease Virus (metabolism)
• HEK293 Cells
• Host-Pathogen Interactions (drug effects)
• Humans
• Mitogen-Activated Protein Kinases (metabolism)
• Receptors, Laminin (metabolism)
• Ribosomal Proteins (metabolism, pharmacology)
• Signal Transduction
• Swine
• Viral Proteins (metabolism)
• Virus Replication