Abstract | BACKGROUND: OBJECTIVES: METHODS:
Thimerosal induced pseudo- allergic reactions via MrgprB2/ MRGPRX2 were investigated using a novel skin pseudo- allergic reaction mouse model, footpad swelling and extravasation assays in vivo and mast cell degranulation assay in vitro. RESULTS:
Thimerosal induced contact dermatitis in dorsal skin and footpad swelling in wild-type mice, but had no significant effect in MrgprB2-knockout mice. Thimerosal-induced dermatitis is characterized by infiltration of inflammatory cells and elevation of serum histamine and inflammatory cytokines, rather than elevation of serum IgE level. Thimerosal increased the intracellular Ca2+ concentration in HEK293 cells overexpressing MrgprB2/MRGPRX2. Downregulation of MRGPRX2 resulted in the reduced degranulation of LAD2 human mast cells. CONCLUSIONS:
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Authors | Bin Peng, Delu Che, Yong Hao, Yi Zheng, Rui Liu, Ye Qian, Jiao Cao, Jue Wang, Yongjing Zhang, Langchong He, Songmei Geng |
Journal | Journal of dermatological science
(J Dermatol Sci)
Vol. 95
Issue 3
Pg. 99-106
(Sep 2019)
ISSN: 1873-569X [Electronic] Netherlands |
PMID | 31558225
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- MRGPRX2 protein, human
- Mrgprb2 protein, mouse
- Nerve Tissue Proteins
- Preservatives, Pharmaceutical
- Receptors, G-Protein-Coupled
- Receptors, Neuropeptide
- Thimerosal
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Topics |
- Administration, Cutaneous
- Animals
- Cell Degranulation
(drug effects)
- Dermatitis, Contact
(etiology, pathology)
- Disease Models, Animal
- HEK293 Cells
- Humans
- Hypersensitivity, Delayed
(etiology, pathology)
- Male
- Mast Cells
(drug effects, pathology)
- Mice
- Mice, Knockout
- Nerve Tissue Proteins
(metabolism)
- Preservatives, Pharmaceutical
(administration & dosage, toxicity)
- Receptors, G-Protein-Coupled
(genetics, metabolism)
- Receptors, Neuropeptide
(metabolism)
- Thimerosal
(administration & dosage, adverse effects)
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