Integrin αvβ3-Specific Hydrocyanine for Cooperative Targeting of Glioblastoma with High Sensitivity and Specificity.

Abstract

Glioblastoma is a highly malignant brain tumor with poor prognosis and survival rate because of a lack of effective diagnostic methods. Hydrocyanines are a type of reactive oxygen species (ROS)-responsive fluorescent probes, allowing for distinguishing tumor cells from normal cells based on their different intracellular levels of ROS. However, their diagnostic applications for glioblastoma have been limited because of the inability to discriminate between tumor cells and other tissues with high ROS production, leading to high false-positive diagnosis. Therefore, tumor-responsive and -specific hydrocyanines with cooperative targeting ability have great potential for improving the diagnosis and treatment of glioblastoma. Integrin αvβ3 plays a critical role in the progression and angiogenesis of glioblastoma and has become a promising target for diagnosing glioblastoma. Herein, we identify a specific peptide ligand for integrin αvβ3, Arg-Trp-(d-Arg)-Asn-Arg (RWrNR), which shows high binding affinity to human glioblastoma U87MG cells. Importantly, hydro-Cy5-RWrNR conjugation allowed for distinguishing U87MG cells from normal cells in response to intracellular ROS. Particularly, hydro-Cy5-RWrNR could not only selectively accumulate in orthotopic U87MG tumor with minimal background fluorescence but also effectively discriminate between glioblastoma and inflammatory tissues for the first time, leading to detection of glioblastoma in vivo with high target-to-background ratios and minimal background fluorescence. Therefore, hydro-Cy5-RWrNR is the first integrin αvβ3-specific hydrocyanine probe and has great potential in precise tumor diagnosis because of its cooperative targeting of integrin αvβ3 and ROS.

• Authors: Lei Zhang, Xia Meng, Xue Shan, Tingting Gu, Jikang Zhang, Shujun Feng, Xueying An, Jiao Chen, Qing Jiang, Xinghai Ning
• Journal: Analytical chemistry (Anal Chem) Vol. 91 Issue 19 Pg. 12587-12595 (10 01 2019) ISSN: 1520-6882 [Electronic] United States
• PMID: 31496223 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carbocyanines
• Fluorescent Dyes
• Integrin alphaVbeta3
• Reactive Oxygen Species

Topics

• Animals
• Carbocyanines (chemistry, metabolism)
• Cell Line, Tumor
• Fluorescent Dyes (chemistry, metabolism)
• Glioblastoma (diagnosis, metabolism, pathology, therapy)
• Humans
• Integrin alphaVbeta3 (metabolism)
• Mice
• Reactive Oxygen Species (metabolism)
• Spheroids, Cellular (metabolism, pathology)