Abstract | BACKGROUND: METHODS: Publicly available databases including Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), Kaplan-Meier plotter, SurvExpress, MethHC, cBioportal, and GeneMANIA were accessed to perform an integrated bioinformatic analysis and try to detect fundamental relationships between each MT family member and GC. RESULTS: Bioinformatic data indicated that the mRNA expression of all MT family members was almost lowly expressed in GC compared with normal gastric tissue (P<0.05), and patients with reduced mRNA expression of each individual MT member had inconsistent prognostic value (OS, FP, PPS), which depended on the individual isoform of MT. A negative correlation between the methylation in promoter region of majority of MT members and their mRNA expression was detected from MethHC database (p<0.001). Data downloaded from TCGA revealed that MTs were rarely mutated in GC patients and MT2A was frequently regulated by other three genes (FOS, JUN, SP1) in GC patients. CONCLUSION: MTs were nearly downregulated, and distinct type of MT harbored different prognostic role in GC patients. Methylation in gene promoter region of MTs partially contributed to their reduced expression in GC. Our comprehensive analyses from multiple independent databases may further lead researches to explore MT-targeting reagents or potential diagnostic and prognostic markers for GC patients.
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Authors | Mingfu Tong, Wenquan Lu, Hao Liu, Jian Wu, Mingzuo Jiang, Xin Wang, Jianyu Hao, Daiming Fan |
Journal | BioMed research international
(Biomed Res Int)
Vol. 2019
Pg. 2957821
( 2019)
ISSN: 2314-6141 [Electronic] United States |
PMID | 31380415
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- RNA, Messenger
- Metallothionein
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Topics |
- Biomarkers, Tumor
(genetics)
- Carcinogenesis
(genetics)
- DNA Methylation
(genetics)
- Disease Progression
- Disease-Free Survival
- Drug Resistance, Neoplasm
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Kaplan-Meier Estimate
- Male
- Metallothionein
(genetics)
- Multigene Family
(genetics)
- Prognosis
- RNA, Messenger
(genetics)
- Stomach Neoplasms
(epidemiology, genetics, pathology)
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