Abstract |
The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; p < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice.
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Authors | Lourdes Santiago-López, Adrián Hernández-Mendoza, Belinda Vallejo-Cordoba, Verónica Mata-Haro, Abraham Wall-Medrano, Aarón F González-Córdova |
Journal | Nutrients
(Nutrients)
Vol. 11
Issue 7
(Jul 16 2019)
ISSN: 2072-6643 [Electronic] Switzerland |
PMID | 31315186
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Cytokines
- Indomethacin
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(toxicity)
- Cytokines
(genetics, metabolism)
- Fermentation
- Gene Expression Regulation
(drug effects)
- Indomethacin
(toxicity)
- Inflammation
(chemically induced, therapy)
- Intestinal Diseases
(chemically induced, therapy)
- Intestines
(drug effects, pathology)
- Kidney
(drug effects, pathology)
- Limosilactobacillus fermentum
(physiology)
- Liver
(drug effects, pathology)
- Mice, Inbred C57BL
- Milk
- Organ Size
- Spleen
(drug effects, pathology)
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