Abstract |
MicroRNAs ( miRNAs) play critical roles in the tumorigenesis and progression of gastric cancer (GC). However, the biological function of miR-4268 in GC and its mechanism remain unclear. In the present study, qTR-PCR found that the expression of miR-4268 was significantly downregulated in GC tissues and cell lines. The overexpression of miR-4268 inhibited GC cell proliferation and the cell cycle G1/S phase transition, and induced cell apoptosis. In contrast, inhibition of miR-4268 promoted cell proliferation and G1-S transition, and suppressed cell apoptosis. Further analyses revealed that miR-4268 expression was negatively correlated with Rab6B expression in GC tissues. Rab6B was verified to be a direct target of miR-4268. Notably, silencing Rab6B resulted in the same biological effects in GC cells as those induced by overexpression of miR-4268. Importantly, both miR-4268 overexpression and Rab6B silence inhibited the AKT/JNK signaling pathways, which modulated cell cycle regulators ( Cyclin D1 and CDK4). In contrast, inhibition of miR-4268 promoted the AKT/JNK signaling pathways. MiR-4268 overexpression also promoted the p38 MAPK signaling pathway. Taken together, miR-4268 suppresses GC cell proliferation through inhibiting the AKT/JNK signaling pathways by targeting Rab6B and induces cell apoptosis through promoting the p38 MAPK signaling pathway. Our findings indicate a tumor-suppressor role of miR-4268 in GC pathogenesis and the potential of miR-4268 in GC theropy.
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Authors | Lingyu Zhao, Meng Xue, Lu Zhang, Bo Guo, Yannan Qin, Qiuyu Jiang, Ruifang Sun, Juang Yang, Lumin Wang, Liying Liu, Xiaofei Wang, Chen Huang, Dongdong Tong |
Journal | Cancer gene therapy
(Cancer Gene Ther)
Vol. 27
Issue 6
Pg. 461-472
(06 2020)
ISSN: 1476-5500 [Electronic] England |
PMID | 31303644
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)
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Chemical References |
- Rab6 protein
- Proto-Oncogene Proteins c-akt
- rab GTP-Binding Proteins
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Topics |
- Apoptosis
(physiology)
- Cell Line, Tumor
- Cell Proliferation
(physiology)
- Humans
- MAP Kinase Signaling System
- Middle Aged
- Proto-Oncogene Proteins c-akt
(metabolism)
- Stomach Neoplasms
(genetics, metabolism, pathology)
- Transfection
- rab GTP-Binding Proteins
(genetics, metabolism)
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