Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells.

Abstract

BACKGROUND:

Psoralen is a coumarin-like and coumarin-related benzofuran glycoside, which is a commonly used traditional Chinese medicine to treat patients with kidney and spleen-yang deficiency symptom. Psoralen has been reported to show estrogen-like activity, antioxidant activity, osteoblastic proliferation accelerating activity, antitumor effects and antibacterial activity. However, the antitumor mechanism of psoralen is not fully understood. This study aimed to investigate the therapeutic efficacy of psoralen in human hepatoma cell line SMMC7721 and the mechanism of antitumor effects.

RESULTS:

Psoralen inhibited proliferation of SMMC7721 in a dose- and time-dependent manner, and promoted apoptosis. Further, psoralen activated the ER stress signal pathway, including the expansion of endoplasmic reticulum, increasing the mRNA levels of GRP78, DDIT3, ATF4, XBP1, GADD34 and the protein levels of GDF15, GRP78, IRE1α, XBP-1s in a time-dependent manner. Psoralen induces cell cycle arrest at G1 phase by enhancing CyclinD1 and reducing CyclinE1 expression. Moreover, TUDC couldn't inhibit the psoralen-induced ER stress in SMMC7721 cells.

CONCLUSIONS:

Psoralen can inhibit the proliferation of SMMC7721 cells and induce ER stress response to induce cell apoptosis, suggesting that psoralen may represent a novel therapeutic option for the prevention and treatment hepatocellular carcinoma.

• Authors: Xiaomin Wang, Peike Peng, Zhiqiang Pan, Zhaoqin Fang, Wenli Lu, Xiaomei Liu
• Journal: Biological research (Biol Res) Vol. 52 Issue 1 Pg. 34 (Jul 05 2019) ISSN: 0717-6287 [Electronic] England
• PMID: 31277690 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Endoplasmic Reticulum Chaperone BiP
• HSPA5 protein, human
• Protein Serine-Threonine Kinases
• Ficusin

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (drug therapy, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Endoplasmic Reticulum Chaperone BiP
• Endoplasmic Reticulum Stress (drug effects)
• Ficusin (chemistry, pharmacology, therapeutic use)
• Humans
• Liver Neoplasms (drug therapy, pathology)
• Protein Serine-Threonine Kinases (pharmacology)
• Signal Transduction (drug effects)