Fermented Oyster Extract Prevents Ovariectomy-Induced Bone Loss and Suppresses Osteoclastogenesis.

Abstract

There is growing interest in bioactive substances from marine organisms for their potential use against diverse human diseases. Osteoporosis is a skeletal disorder associated with bone loss primarily occurring through enhanced osteoclast differentiation and resorption. Recently, we reported the anti-osteoclastogenic activity of fermented Pacific oyster (Crassostrea gigas) extract (FO) in vitro. The present study focused on investigating the anti-osteoporotic efficacy of FO in bone loss prevention in an experimental animal model of osteoporosis and elucidating the mechanism underlying its effects. Oral administration of FO significantly decreased ovariectomy-induced osteoclast formation and prevented bone loss, with reduced serum levels of bone turnover biomarkers including osteocalcin and C-terminal telopeptide fragment of type I collagen C-terminus (CTX). FO significantly suppressed receptor activator of nuclear factor-κB ligand (RANKL)-induced differentiation of bone marrow-derived macrophages (BMMs) into osteoclasts and attenuated the induction of osteoclast-specific genes required for osteoclastogenesis and bone resorption. Furthermore, FO inhibited RANKL-mediated IκBα and p65 phosphorylation in BMMs. Taken together, these results demonstrate that FO effectively suppresses osteoclastogenesis in vivo and in vitro, and that FO can be considered as a potential therapeutic option for the treatment of osteoporosis and osteoclast-mediated skeletal diseases.

• Authors: Hye Jung Ihn, Ju Ang Kim, Soomin Lim, Sang-Hyeon Nam, So Hyeon Hwang, Jiwon Lim, Gi-Young Kim, Yung Hyun Choi, You-Jin Jeon, Bae-Jin Lee, Jong-Sup Bae, Yeo Hyang Kim, Eui Kyun Park
• Journal: Nutrients (Nutrients) Vol. 11 Issue 6 (Jun 21 2019) ISSN: 2072-6643 [Electronic] Switzerland
• PMID: 31234292 (Publication Type: Journal Article)

Chemical References

• Actins
• Bone Density Conservation Agents
• NF-kappa B

Topics

• Actins (metabolism)
• Animals
• Bone Density Conservation Agents (isolation & purification, pharmacology)
• Cells, Cultured
• Crassostrea (microbiology)
• Disease Models, Animal
• Female
• Fermentation
• Humans
• Levilactobacillus brevis (physiology)
• Mice, Inbred ICR
• NF-kappa B (metabolism)
• Osteoclasts (drug effects, metabolism, pathology)
• Osteogenesis (drug effects)
• Osteoporosis, Postmenopausal (metabolism, pathology, physiopathology, prevention & control)
• Ovariectomy
• Seafood (microbiology)
• Signal Transduction
• Tibia (drug effects, metabolism, pathology, physiopathology)