Therapeutic Cell Protective Role of Histochrome under Oxidative Stress in Human Cardiac Progenitor Cells.

Abstract

Cardiac progenitor cells (CPCs) are resident stem cells present in a small portion of ischemic hearts and function in repairing the damaged heart tissue. Intense oxidative stress impairs cell metabolism thereby decreasing cell viability. Protecting CPCs from undergoing cellular apoptosis during oxidative stress is crucial in optimizing CPC-based therapy. Histochrome (sodium salt of echinochrome A-a common sea urchin pigment) is an antioxidant drug that has been clinically used as a pharmacologic agent for ischemia/reperfusion injury in Russia. However, the mechanistic effect of histochrome on CPCs has never been reported. We investigated the protective effect of histochrome pretreatment on human CPCs (hCPCs) against hydrogen peroxide (H2O2)-induced oxidative stress. Annexin V/7-aminoactinomycin D (7-AAD) assay revealed that histochrome-treated CPCs showed significant protective effects against H2O2-induced cell death. The anti-apoptotic proteins B-cell lymphoma 2 (Bcl-2) and Bcl-xL were significantly upregulated, whereas the pro-apoptotic proteins BCL2-associated X (Bax), H2O2-induced cleaved caspase-3, and the DNA damage marker, phosphorylated histone (γH2A.X) foci, were significantly downregulated upon histochrome treatment of hCPCs in vitro. Further, prolonged incubation with histochrome alleviated the replicative cellular senescence of hCPCs. In conclusion, we report the protective effect of histochrome against oxidative stress and present the use of a potent and bio-safe cell priming agent as a potential therapeutic strategy in patient-derived hCPCs to treat heart disease.

• Authors: Ji Hye Park, Na-Kyung Lee, Hye Ji Lim, Sinthia Mazumder, Vinoth Kumar Rethineswaran, Yeon-Ju Kim, Woong Bi Jang, Seung Taek Ji, Songhwa Kang, Da Yeon Kim, Le Thi Hong Van, Ly Thanh Truong Giang, Dong Hwan Kim, Jong Seong Ha, Jisoo Yun, Hyungtae Kim, Jin Han, Natalia P Mishchenko, Sergey A Fedoreyev, Elena A Vasileva, Sang Mo Kwon, Sang Hong Baek
• Journal: Marine drugs (Mar Drugs) Vol. 17 Issue 6 (Jun 21 2019) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 31234277 (Publication Type: Journal Article)

Chemical References

• Annexin A5
• Apoptosis Regulatory Proteins
• Naphthoquinones
• bcl-2-Associated X Protein
• histochrome
• Hydrogen Peroxide
• Caspase 3
• echinochrome A

Topics

• Annexin A5 (metabolism)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (metabolism)
• Caspase 3 (metabolism)
• Cell Survival (drug effects)
• Cells, Cultured
• Cellular Senescence (drug effects)
• DNA Damage (drug effects)
• Humans
• Hydrogen Peroxide (pharmacology)
• Myocytes, Cardiac (drug effects, metabolism)
• Naphthoquinones (pharmacology)
• Oxidative Stress (drug effects)
• Reperfusion Injury (chemically induced, drug therapy, metabolism)
• Russia
• Stem Cells (drug effects)
• bcl-2-Associated X Protein (metabolism)