Abstract | BACKGROUND: METHODS: Aqueous humour samples were collected from nine PDR eyes and nine cataract control eyes, and NGS was performed. Quantitative polymerase chain reaction (qPCR) was used to validate the sequencing results. An oxygen-induced retinopathy (OIR) model was used to validate the angiogenesis related miRNA. RESULTS: In total, 484 miRNAs were differently expressed between the PDR eyes and cataract control eyes, including 210 mature miRNAs and 274 novel miRNAs. Furthermore, eight miRNAs and 30 piRNAs were identified as the most differently expressed between the two groups (P > .85). This differential expression of miRNA was predicted to regulate Rho protein signal transduction, neurotransmitter uptake and histone lysine methylation. Relative expression patterns of miR-184, -150-5p and -93-5p were confirmed by qPCR. A reduced expression of miR-93-5p was confirmed in the OIR model. CONCLUSIONS: This study comprehensively demonstrated the miRNA and piRNA expression profile of the aqueous humour of PDR eyes, which may serve as a potential biomarker and involved in the pathogenesis of PDR.
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Authors | Shida Chen, Miner Yuan, Yaoming Liu, Xiujuan Zhao, Ping Lian, Yang Chen, Bingqian Liu, Lin Lu |
Journal | Clinical & experimental ophthalmology
(Clin Exp Ophthalmol)
Vol. 47
Issue 7
Pg. 925-936
(Sep 2019)
ISSN: 1442-9071 [Electronic] Australia |
PMID | 31081578
(Publication Type: Journal Article)
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Copyright | © 2019 Royal Australian and New Zealand College of Ophthalmologists. |
Chemical References |
- Genetic Markers
- MicroRNAs
- RNA, Small Interfering
- RNA, Untranslated
|
Topics |
- Aged
- Animals
- Animals, Newborn
- Aqueous Humor
(metabolism)
- Diabetic Retinopathy
(metabolism)
- Disease Models, Animal
- Female
- Gene Expression Profiling
- Genetic Markers
- High-Throughput Nucleotide Sequencing
- Humans
- Male
- Mice
- Mice, Inbred C57BL
- MicroRNAs
(metabolism)
- Middle Aged
- RNA, Small Interfering
(metabolism)
- RNA, Untranslated
(metabolism)
- Real-Time Polymerase Chain Reaction
- Retinal Neovascularization
(metabolism)
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