Abstract | BACKGROUND:
Ischemia/reperfusion injury (IRI) can occur during liver surgery. Endogenous catalase is important to cellular antioxidant defenses and is critical to IRI prevention. Pegylation of catalase ( PEG-CAT) improves its therapeutic potential by extending plasma half-life, but systemic administration of exogenous PEG-CAT has been only mildly therapeutic for hepatic IRI. Here, we investigated the protective effects of direct intrahepatic delivery of PEG-CAT during IRI using a rat hilar clamp model. MATERIALS AND METHODS: RESULTS: In vitro, PEG-CAT pretreatment of liver cells showed substantial uptake and protection against oxidative stress injury. In vivo, direct intrahepatic, but not systemic, delivery of PEG-CAT during IRI significantly reduced alanine aminotransferase and aspartate aminotransferase in a time-dependent manner (P < 0.01, P < 0.0001, respectively, for all time points) compared to control. Similarly, tissue malondialdehyde (P = 0.0048), adenosine triphosphate (P = 0.019), and GSH (P = 0.0015), and the degree of centrilobular necrosis, were improved by intrahepatic compared to systemic PEG-CAT delivery. CONCLUSIONS: Direct intrahepatic administration of PEG-CAT achieved significant protection against IRI by reducing the volume distribution and taking advantage of the substantial hepatic first-pass uptake of this molecule. The mode of delivery was an important factor for protection against hepatic IRI by PEG-CAT.
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Authors | Clifford Akateh, Eliza W Beal, Jung-Lye Kim, Brenda F Reader, Katelyn Maynard, Jay L Zweier, Bryan A Whitson, Sylvester M Black |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 238
Pg. 152-163
(06 2019)
ISSN: 1095-8673 [Electronic] United States |
PMID | 30771685
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2019 Elsevier Inc. All rights reserved. |
Chemical References |
- catalase-polyethylene glycol
- Polyethylene Glycols
- Hydrogen Peroxide
- Catalase
- Aspartate Aminotransferases
- Alanine Transaminase
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Topics |
- Alanine Transaminase
(blood)
- Animals
- Aspartate Aminotransferases
(blood)
- Catalase
(administration & dosage)
- Cell Survival
(drug effects)
- Disease Models, Animal
- Humans
- Hydrogen Peroxide
(pharmacology)
- Injections, Intralesional
- Liver
(blood supply, cytology, surgery)
- Male
- Oxidative Stress
(drug effects)
- Polyethylene Glycols
(administration & dosage)
- Primary Cell Culture
- Rats
- Reperfusion Injury
(blood, drug therapy, etiology)
- Treatment Outcome
- Warm Ischemia
(adverse effects)
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