Induction of herpes simplex virus type 1 cell-to-cell spread inhibiting antibodies by a calcium phosphate nanoparticle-based vaccine.

Abstract

Herpes simplex viruses 1 and 2 are among the most ubiquitous human infections and persist lifelong in their host. Upon primary infection or reactivation from ganglia, the viruses spread by direct cell-cell contacts (cell-to-cell spread) and thus escape from the host immune response. We have developed a monoclonal antibody (mAb 2c), which inhibits the HSV cell-to-cell spread, thereby protecting from lethal genital infection and blindness in animal models. In the present study we have designed a nanoparticle-based vaccine to induce protective antibody responses exceeding the cell-to-cell spread inhibiting properties of mAb 2c. We used biodegradable calcium phosphate (CaP) nanoparticles coated with a synthetic peptide that represents the conformational epitope on HSV-1 gB recognized by mAb 2c. The CaP nanoparticles additionally contained a TLR-ligand CpGm and were formulated with adjuvants to facilitate the humoral immune response. This vaccine effectively protected mice from lethal HSV-1 infection by inducing cell-to-cell spread inhibiting antibodies.

• Authors: Mathis Kopp, Ulrich Wilhelm Aufderhorst, Mira Alt, Ulf Dittmer, Anna-Maria Eis-Hübinger, Bernd Giebel, Michael Roggendorf, Matthias Epple, Adalbert Krawczyk
• Journal: Nanomedicine : nanotechnology, biology, and medicine (Nanomedicine) Vol. 16 Pg. 138-148 (02 2019) ISSN: 1549-9642 [Electronic] United States
• PMID: 30594660 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Calcium Phosphates
• Herpesvirus Vaccines
• calcium phosphate

Topics

• Animals
• Antibodies, Monoclonal (chemistry, therapeutic use)
• Antibodies, Viral (chemistry, therapeutic use)
• Calcium Phosphates (chemistry)
• Chlorocebus aethiops
• Female
• Herpesvirus 1, Human (immunology, pathogenicity)
• Herpesvirus Vaccines (chemistry, immunology, therapeutic use)
• Mice
• Mice, Inbred BALB C
• Nanoparticles (chemistry, therapeutic use)
• Vero Cells