Lactobacillus plantarum YS2 (yak yogurt Lactobacillus) exhibited an activity to attenuate activated carbon-induced constipation in male Kunming mice.

Abstract

The present study investigated the effects of Lactobacillus plantarum YS2 (LP-YS2) that was isolated from yak yogurt on activated carbon-induced constipation in Kunming (KM) mice. The KM mice were orally administered LP-YS2 and reference strain Lactobacillus delbrueckii ssp. bulgaricus. Administration of LP-YS2 [1.0 × 109 cfu/kg of body weight (BW)] promoted gastrointestinal peristalsis and reduced the first black stool defecation time (129 min), which clearly defines attenuation of the voiding difficulty in mice with constipation. The LP-YS2 treatment also increased the serum level of motilin (MTL; 178.2 pg/mL), gastrin (69.4 pg/mL), acetylcholine (Ach; 30.1 pg/mL), substance P (SP; 57.6 pg/mL), and vasoactive intestinal peptide (VIP; 53.2 pg/mL) and reduced the somatostatin (SS, 32.6 pg/mL) levels compared with the L. delbrueckii ssp. bulgaricus treatment (MTL, 139.7 pg/mL; gastrin, 43.1 pg/mL; Ach, 15.9 pg/mL; SP, 43.6 pg/mL; VIP, 32.3 pg/mL; SS, 55.1 pg/mL) and the control (MTL, 105.3 pg/mL; gastrin, 26.7 pg/mL; Ach, 9.7 pg/mL; SP, 30.2 pg/mL; VIP, 21.0 pg/mL; SS, 70.5 pg/mL). The LP-YS2 treatment significantly increased the colonic mRNA and protein expression of c-Kit (CD117, cluster of differentiation 117; 2.87 times mRNA expression of the control group), stem cell factor (30.40 times mRNA expression of the control group), and glial cell-derived neurotrophic factor (29.97 times mRNA expression of the control group) in mice with constipation. In addition, LP-YS2 reduced the expression of transient receptor potential vanilloid 1 (0.42 times mRNA expression of the control group) and nitric oxide synthase (0.49 times mRNA expression of the control group) in constipated mice. These results demonstrate that LP-YS2 was able to attenuate the activated carbon-induced constipation in KM mice.

• Authors: Xin Zhao, Yu Qian, Guijie Li, Ruokun Yi, Kun-Young Park, Jia-Le Song
• Journal: Journal of dairy science (J Dairy Sci) Vol. 102 Issue 1 Pg. 26-36 (Jan 2019) ISSN: 1525-3198 [Electronic] United States
• PMID: 30527985 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Biomarkers
• Charcoal
• Substance P
• Nitric Oxide Synthase

Topics

• Animals
• Antioxidants (analysis)
• Biomarkers (analysis)
• Body Weight
• Cattle
• Charcoal (adverse effects)
• Colon (metabolism)
• Constipation (chemically induced, prevention & control, therapy)
• Defecation
• Gastrointestinal Tract (metabolism)
• Gastrointestinal Transit (physiology)
• Intestine, Small (metabolism)
• Lactobacillus delbrueckii (metabolism)
• Lactobacillus plantarum (metabolism)
• Male
• Mice
• Nitric Oxide Synthase (metabolism)
• Peristalsis (physiology)
• Substance P (metabolism)
• Yogurt (microbiology)