A black phosphorus/manganese dioxide nanoplatform: Oxygen self-supply monitoring, photodynamic therapy enhancement and feedback.

Abstract

Selecting the timing of laser treatment is an important task for improving O2-dependent photodynamic therapy (PDT) efficiency. Here, a black phosphorus-based strategy was developed for dual-mode monitoring oxygen self-supply, enhancing photodynamic therapy, and feeding back therapeutic effect. The hybridized nanoplatform (R-MnO2-FBP) was prepared by assembly of Rhodamine B (RhB)-encapsulated manganese dioxide (R-MnO2) as O2 supplier and indicator, and fluorescein isothiocyanate (FITC)-labelled peptide-functionalized black phosphorus as the theranostic agent. The time-dependent assays suggested that the O2 release was proportional to the liberation of Mn2+ and RhB in the R-MnO2-FBP system. After specific delivery into cancer cells, R-MnO2-FBP was dissociated in the acidic and H2O2-rich environment and generated oxygen to overcome hypoxia-associated PDT resistance. In the meantime, it released both Mn2+and RhB dye, leading to dual-mode (magnetic resonance imaging/fluorescence imaging) monitoring of the oxygen self-supply process. More significantly, the imaging-guided PDT in hypoxic cells displayed 51.6% of cell apoptosis at optimizing timing of laser application, which could also be confirmed by the FITC fluorescence recovery induced by the activated caspase-3 in apoptotic cells. In vivo photonic therapy by R-MnO2-FBP further demonstrated the ability of R-MnO2-FBP to choose the timing of laser application, providing an efficient approach for the enhancement of PDT process.

• Authors: Jintong Liu, Ping Du, Tianrui Liu, Bernardino J Córdova Wong, Weiping Wang, Huangxian Ju, Jianping Lei
• Journal: Biomaterials (Biomaterials) Vol. 192 Pg. 179-188 (02 2019) ISSN: 1878-5905 [Electronic] Netherlands
• PMID: 30453214 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.

Chemical References

• Fluorescent Dyes
• Manganese Compounds
• Oxides
• Photosensitizing Agents
• Phosphorus
• Oxygen
• manganese dioxide

Topics

• Animals
• Female
• Fluorescent Dyes (chemistry, therapeutic use)
• HeLa Cells
• Humans
• Magnetic Resonance Imaging
• Manganese Compounds (chemistry, therapeutic use)
• Mice, Inbred BALB C
• Mice, Nude
• Nanoparticles (chemistry, therapeutic use, ultrastructure)
• Neoplasms (diagnostic imaging, therapy)
• Optical Imaging
• Oxides (chemistry, therapeutic use)
• Oxygen (analysis)
• Phosphorus (chemistry, therapeutic use)
• Photochemotherapy
• Photosensitizing Agents (chemistry, therapeutic use)
• Theranostic Nanomedicine