Effect of Radix isatidis polysaccharide on alleviating insulin resistance in type 2 diabetes mellitus cells and rats.

Abstract

OBJECTIVES:

The objective of this paper was to explore the effects of Radix isatidis polysaccharide (RIP) extracted from Radix isatis on alleviating insulin resistance.

METHODS:

The insulin resistance models of 3T3-L1 preadipocytes and type 2 diabetic rats were established to evaluate the insulin resistance activity of RIP.

KEY FINDINGS:

Radix isatidis polysaccharide within the concentration range of 25-100 μg/ml could reduce cell supernatant glucose and TNF-α levels (P < 0.01) and increase the expression of PI-3K P85, Glut4, IRS-1 and Akt protein in symptoms of IR 3T3-L1 preadipocytes. In the meantime, RIP contributed to relieve the weight loss of diabetic rats whose liver weight and liver index were decreased due to the effects of RIP. Experiments in rats also showed that RIP had capacity in reduced serum TC, TG, LDL-C, FFA, FBG, FINS, MDA, ALT, AST activities and increased serum HDL-C, SOD, ISI (P < 0.05 or 0.01). In addition, the oral glucose tolerance in rats was improved (P < 0.05) and liver damage was restored due to RIP.

CONCLUSIONS:

Radix isatidis polysaccharide significantly alleviates insulin resistance in 3T3-L1 preadipocytes and type 2 diabetic rats. These beneficial effects of RIP may associate with their roles in improving the glucose metabolism, lipid metabolism and oxidative stress.

• Authors: Ji-Ping Li, Ye Yuan, Wen-You Zhang, Zhen Jiang, Tian-Jiao Hu, Yi-Ting Feng, Mo-Xiang Liu
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 71 Issue 2 Pg. 220-229 (Feb 2019) ISSN: 2042-7158 [Electronic] England
• PMID: 30298631 (Publication Type: Journal Article)
• Copyright: © 2018 Royal Pharmaceutical Society.

Chemical References

• Blood Glucose
• Drugs, Chinese Herbal
• Polysaccharides
• banlangen

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism)
• Animals
• Blood Glucose (drug effects)
• Diabetes Mellitus, Experimental (drug therapy)
• Diabetes Mellitus, Type 2 (drug therapy)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (administration & dosage, chemistry, pharmacology)
• Glucose Tolerance Test
• Insulin Resistance
• Lipid Metabolism (drug effects)
• Male
• Mice
• Oxidative Stress (drug effects)
• Plant Roots
• Polysaccharides (administration & dosage, isolation & purification, pharmacology)
• Rats
• Rats, Wistar