Proteomic analysis serves as an important biological tool for identifying biological events. Novel
biomarkers of a specific disease such as
cancer may be identified using these promising techniques. The aim of the present study was to investigate the effect of
tangeretin and to identify potential
biomarkers in AGS
gastric cancer cells using a proteomics approach. The results of the present study revealed that
tangeretin inhibited AGS cell viability dose‑dependently with a half‑maximal inhibitory concentration of 100 µM. Two‑dimensional gel electrophoresis was performed to determine the potential
biomarker between control and
tangeretin (100 µM)‑treated AGS cells. A total of 16 proteins was identified from 36 significant
protein spots using matrix‑assisted laser‑desorption/ionization time‑of‑flight‑mass spectrometry using
peptide fingerprinting. The bioinformatics tools
Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to identify the functional properties and association of the
proteins obtained. Using western blot analysis, the regulatory pattern of four selected
proteins,
protein kinase Cε, mitogen‑activated
protein kinase 4, phosphoinositide 4‑kinase and poly(ADP‑ribose) polymerase 14, were successfully verified in replicate sample sets. These selected
proteins are primarily involved in apoptosis signaling, angiogenesis, cell cycle regulation, receptor
kinase binding, intracellular cytoplasmic and nuclear alterations. Therefore, aim of the present study was to identify potential diagnostic
biomarkers from the functional categories of altered
protein expression in tangeretin‑inhibited AGS
gastric cancer cell viability.