A simultaneous oral and intramuscular prime/sublingual boost with a DNA/Modified Vaccinia Ankara viral vector-based vaccine induces simian immunodeficiency virus-specific systemic and mucosal immune responses in juvenile rhesus macaques.

Abstract

BACKGROUND:

A pediatric vaccine to prevent breast milk transmission of human immunodeficiency virus (HIV) may generate greater immune responses at viral entry sites if given by an oral route.

METHODS:

We compared immune responses induced in juvenile macaques by prime/boosting with simian immunodeficiency virus (SIV)-expressing DNA/modified vaccinia Ankara virus (MVA) by the intramuscular route (IM), the oral (O)/tonsillar routes (T), the O/sublingual (SL) routes, and O+IM/SL routes.

RESULTS:

O/T or O/SL immunization generated SIV-specific T cells in mucosal tissues but failed to induce SIV-specific IgA in saliva or stool or IgG in plasma. IM/IM or O+IM/SL generated humoral and cellular responses to SIV. IM/IM generated greater frequencies of TFH in spleen, but O+IM/SL animals had higher avidity plasma IgG and more often demonstrated mucosal IgA responses.

CONCLUSION:

These results suggest that codelivery of HIV DNA/MVA vaccines by the oral and IM routes might be optimal for generating both systemic and mucosal antibodies.

• Authors: Alan D Curtis 2nd, Kara Jensen, Koen K A Van Rompay, Rama R Amara, Pamela A Kozlowski, Kristina De Paris
• Journal: Journal of medical primatology (J Med Primatol) Vol. 47 Issue 5 Pg. 288-297 (10 2018) ISSN: 1600-0684 [Electronic] Denmark
• PMID: 30204253 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• DNA, Viral
• SAIDS Vaccines

Topics

• Administration, Oral
• Administration, Sublingual
• Animals
• DNA, Viral (adverse effects)
• Immunity, Cellular (immunology)
• Immunity, Mucosal (immunology)
• Immunogenicity, Vaccine (immunology)
• Injections, Intramuscular (adverse effects)
• Macaca mulatta
• Monkey Diseases (immunology)
• Proof of Concept Study
• SAIDS Vaccines (adverse effects)
• Simian Immunodeficiency Virus (immunology)
• Vaccinia (immunology)