Imaging the interaction of αv integrin-GFP in osteosarcoma cells with RFP-expressing host stromal cells and tumor-scaffold collagen in the primary and metastatic tumor microenvironment.

Abstract

Human osteosarcoma 143B cells were previously stably transfected with an αv integrin green flourescent protein (GFP) vector. 143B cells expressing αv integrin-GFP were transplanted orthotopically in the tibia of transgenic nude mice ubiquitously expressing red fluorescent protein (RFP). The primary tumors acquired RFP-expressing stroma and were passaged orthotopically in the tibia in noncolored nude mice, which maintained the RFP stroma. The interaction of αv integrin-GFP expression in 143B cells with RFP-expressing host stromal cells was observed by confocal microscopy using the Olympus FV1000. Collagen fibers were imaged simultaneously in reflectance mode. The RFP-expressing stroma included cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs) which persisted even 3 weeks after passage to nontransgenic nude mice. CAFs expressing RFP were aligned between collagen fibers and cancer cells expressing αv integrin-GFP. Six weeks after transplantation, pulmonary metastases expressing αv integrin-GFP could be identified. TAMs expressing RFP accompanied metastasized osteosarcoma cells expressing αv integrin-GFP in the lung. The current study demonstrates the importance of αv integrin interaction with stromal elements in osteosarcoma.

• Authors: Yasunori Tome, Tasuku Kiyuna, Fuminari Uehara, Michael Bouvet, Hiroyuki Tsuchiya, Fuminori Kanaya, Robert M Hoffman
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 120 Issue 1 Pg. 283-289 (01 2019) ISSN: 1097-4644 [Electronic] United States
• PMID: 30145815 (Publication Type: Journal Article)
• Copyright: © 2018 Wiley Periodicals, Inc.

Chemical References

• Integrin alphaV
• Luminescent Proteins
• red fluorescent protein
• Green Fluorescent Proteins
• Collagen

Topics

• Animals
• Bone Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Collagen (metabolism)
• Fibroblasts (metabolism)
• Green Fluorescent Proteins (genetics, metabolism)
• Humans
• Integrin alphaV (genetics, metabolism)
• Luminescent Proteins (metabolism)
• Lung Neoplasms (metabolism, secondary)
• Macrophages (metabolism)
• Male
• Mice
• Mice, Nude
• Mice, Transgenic
• Microscopy, Confocal
• Osteosarcoma (metabolism, pathology)
• Stromal Cells (metabolism)
• Transfection
• Transplantation, Heterologous
• Tumor Microenvironment