Abstract |
Cisplatin (CP) is one of the most effective and widely used compounds in the treatment of disease, including cancer, but is known to induce toxicity in patients. Rutin (RUT) is a flavonoid glycoside from Sophora japonica L. that has been shown to possess antioxidative, anti-inflammatory, and antiviral properties. RUT is also known to attenuate cardiotoxicity, isoproterenol-induced cardiac fibrosis, and ischemia/reperfusion-associated hemodynamic alteration, and prevents high glucose-induced renal glomerular endothelial hyperpermeability. In this study, we investigated the effect of RUT on CP-induced nephrotoxicity. CP was used to induce toxicity in human mesangial cells (HMCs), HMCs were pretreated with different concentrations of RUT before being exposed to 10 μg/mL of CP. A positive group was pretreated with antioxidant agent N-acetylcysteine prior to CP administration. At doses between 12.5 and 25 μM, RUT prevented CP-induced reduction in cell viability. Treatment with RUT suppressed intracellular reactive oxygen species and malonic dialdehyde levels and inhibited cell apoptosis. RUT reversed the CP-induced upregulation of p53, cleaved-caspase-3, and increased pro-caspase-3 and pro-caspase-9 levels. In conclusion, the RUT can relieve CP-induced nephrotoxicity by inhibiting the p53/ caspase signaling pathway.
|
Authors | Y Zhang, Q Wang, Y-D Wang, B Sun, X-W Leng, Q Li, L-Q Ren |
Journal | Human & experimental toxicology
(Hum Exp Toxicol)
Vol. 38
Issue 1
Pg. 118-128
(Jan 2019)
ISSN: 1477-0903 [Electronic] England |
PMID | 29962303
(Publication Type: Journal Article)
|
Chemical References |
- Antineoplastic Agents
- Protective Agents
- Reactive Oxygen Species
- Tumor Suppressor Protein p53
- Rutin
- CASP3 protein, human
- CASP9 protein, human
- Caspase 3
- Caspase 9
- Cisplatin
|
Topics |
- Antineoplastic Agents
(toxicity)
- Apoptosis
(drug effects)
- Caspase 3
(metabolism)
- Caspase 9
(metabolism)
- Cells, Cultured
- Cisplatin
(toxicity)
- Humans
- Mesangial Cells
(drug effects, metabolism)
- Protective Agents
(pharmacology)
- Reactive Oxygen Species
(metabolism)
- Rutin
(pharmacology)
- Signal Transduction
(drug effects)
- Tumor Suppressor Protein p53
(metabolism)
|