Aluminum fluoride-18 labeled folate enables in vivo detection of atherosclerotic plaque inflammation by positron emission tomography.

Abstract

Inflammation plays an important role in the development of atherosclerosis and its complications. Because the folate receptor β (FR-β) is selectively expressed on macrophages, an FR targeted imaging agent could be useful for assessment of atherosclerotic inflammation. We investigated aluminum fluoride-18-labeled 1,4,7-triazacyclononane-1,4,7-triacetic acid conjugated folate (18F-FOL) for the detection of atherosclerotic plaque inflammation. We studied atherosclerotic plaques in mice, rabbits, and human tissue samples using 18F-FOL positron emission tomography/computed tomography (PET/CT). Compound 2-deoxy-2-[18F]fluoro-D-glucose (18F-FDG) was used as a comparison. Firstly, we found that the in vitro binding of 18F-FOL co-localized with FR-β-positive macrophages in carotid endarterectomy samples from patients with recent ischemic symptoms. We then demonstrated specific accumulation of intravenously administered 18F-FOL in atherosclerotic plaques in mice and rabbits using PET/CT. We noticed that the 18F-FOL uptake correlated with the density of macrophages in plaques and provided a target-to-background ratio as high as 18F-FDG, but with considerably lower myocardial uptake. Thus, 18F-FOL PET/CT targeting of FR-β-positive macrophages presents a promising new tool for the in vivo imaging of atherosclerotic inflammation.

• Authors: Johanna M U Silvola, Xiang-Guo Li, Jenni Virta, Päivi Marjamäki, Heidi Liljenbäck, Jarkko P Hytönen, Miikka Tarkia, Virva Saunavaara, Saija Hurme, Senthil Palani, Harri Hakovirta, Seppo Ylä-Herttuala, Pekka Saukko, Qingshou Chen, Philip S Low, Juhani Knuuti, Antti Saraste, Anne Roivainen
• Journal: Scientific reports (Sci Rep) Vol. 8 Issue 1 Pg. 9720 (06 26 2018) ISSN: 2045-2322 [Electronic] England
• PMID: 29946129 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aluminum Compounds
• Fluorodeoxyglucose F18
• Fluorides
• aluminum fluoride

Topics

• Aluminum Compounds (chemistry)
• Animals
• Female
• Fluorides (chemistry)
• Fluorodeoxyglucose F18 (chemistry)
• Humans
• Inflammation (diagnostic imaging)
• Male
• Mice
• Plaque, Atherosclerotic (diagnostic imaging)
• Positron Emission Tomography Computed Tomography (methods)
• Rabbits