Nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus DNA fragment capable of trans-inducing alpha genes.

Abstract

The five alpha genes of herpes simplex virus 1 are the first set of genes to be expressed after infection. Previous studies have shown that alpha genes resident in eukaryotic cells are induced by infection with herpes simplex virus 1 or 2 but not by other herpesviruses and indicate that the alpha trans-inducing factor was a structural component of the virion. This factor induces genes linked to a bona fide promoter and containing at the 5' end a small sequence derived from the promoter-regulatory domains of alpha genes. We report the sequence of a small DNA fragment shown previously to be capable of expressing the alpha trans-inducing factor in transient expression systems. The only gene encoded in its entirety in this fragment is predicted to specify a 479 amino acid protein with a Mr of 53,053. The precise termini of the 1.74-kilobase mRNA specifying this protein were determined in our 5' and 3' S1 nuclease protection studies.

• Authors: P E Pellett, J L McKnight, F J Jenkins, B Roizman
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 82 Issue 17 Pg. 5870-4 (Sep 1985) ISSN: 0027-8424 [Print] United States
• PMID: 2994050 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Viral
• Transcription Factors
• Viral Proteins
• Thymidine Kinase

Topics

• Amino Acid Sequence
• Base Sequence
• DNA, Viral (genetics)
• Enzyme Induction
• Genetic Linkage
• Molecular Weight
• Protein Biosynthesis
• Simplexvirus (genetics)
• Thymidine Kinase (genetics)
• Transcription Factors (genetics)
• Transcription, Genetic
• Viral Proteins (genetics)