Abstract | BACKGROUND/AIMS: MATERIALS AND METHODS: Fifty adult guinea pigs were randomly divided into five groups: a sham-administered group (control group); two groups that were intraperitoneally administered an anti-polyclonal neutrophil (PMN) antibody 24 h before common bile duct ligation (CBDL); and two groups of guinea pigs that were subjected to CBDL without receiving the PMN antibody. Guinea pigs that underwent CBDL were held for 24 h or 48 h after surgery before being subjected to laparotomy and cholecystectomy. Immunohistochemistry, TUNEL assays, western blotting, and real-time PCR were performed to determine ICC morphology and density, to detect ICC apoptosis, and to examine stem cell factor (SCF) and c-kit protein expression and SCF and c-kit mRNA levels, respectively. RESULTS: CONCLUSIONS:
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Authors | Zhen-Peng Huang, Hu Qiu, Bao-Ping Yu |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 47
Issue 2
Pg. 535-544
( 2018)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 29794438
(Publication Type: Journal Article)
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Copyright | © 2018 The Author(s). Published by S. Karger AG, Basel. |
Chemical References |
- Antibodies
- RNA, Messenger
- Stem Cell Factor
- Proto-Oncogene Proteins c-kit
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Topics |
- Animals
- Antibodies
(administration & dosage)
- Apoptosis
- Blotting, Western
- Cholecystitis, Acute
(metabolism, pathology)
- Disease Models, Animal
- Female
- Gallbladder
(metabolism, pathology)
- Guinea Pigs
- Immunohistochemistry
- Interstitial Cells of Cajal
(cytology, metabolism)
- Male
- Neutrophils
(immunology)
- Proto-Oncogene Proteins c-kit
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Real-Time Polymerase Chain Reaction
- Stem Cell Factor
(genetics, metabolism)
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