Development of muscular dystrophy in a CRISPR-engineered mutant rabbit model with frame-disrupting ANO5 mutations.

Abstract

Limb girdle muscular dystrophy type 2L (LGMD2L) and Miyoshi myopathy type 3 (MMD3) are autosomal recessive muscular dystrophy caused by mutations in the gene encoding anoctamin-5 (ANO5), which belongs to the anoctamin protein family. Two independent lines of mice with complete disruption of ANO5 transcripts did not exhibit overt muscular dystrophy phenotypes; instead, one of these mice was observed to present with some abnormality in sperm motility. In contrast, a third line of ANO5-knockout (KO) mice with residual expression of truncated ANO5 expression was reported to display defective membrane repair and very mild muscle pathology. Many of the ANO5-related patients carry point mutations or small insertions/deletions (indels) in the ANO5 gene. To more closely mimic the human ANO5 mutations, we engineered mutant ANO5 rabbits via co-injection of Cas9 mRNA and sgRNA into the zygotes. CRISPR-mediated small indels in the exon 12 and/or 13 in the mutant rabbits lead to the development of typical signs of muscular dystrophy with increased serum creatine kinase (CK), muscle necrosis, regeneration, fatty replacement and fibrosis. This novel ANO5 mutant rabbit model would be useful in studying the disease pathogenesis and therapeutic treatments for ANO5-deficient muscular dystrophy.

• Authors: Tingting Sui, Li Xu, Yeh Siang Lau, Di Liu, Tingjun Liu, Yandi Gao, Liangxue Lai, Renzhi Han, Zhanjun Li
• Journal: Cell death & disease (Cell Death Dis) Vol. 9 Issue 6 Pg. 609 (05 22 2018) ISSN: 2041-4889 [Electronic] England
• PMID: 29789544 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Anoctamins
• Cardiotoxins

Topics

• Animals
• Anoctamins (genetics)
• Base Sequence
• Cardiotoxins (toxicity)
• Clustered Regularly Interspaced Short Palindromic Repeats (genetics)
• Disease Models, Animal
• Embryo, Mammalian (drug effects, metabolism)
• Genetic Engineering
• Muscles (drug effects, pathology, physiopathology)
• Muscular Dystrophy, Animal (genetics, pathology, physiopathology)
• Mutation (genetics)
• Phenotype
• Rabbits
• Regeneration (drug effects)