Chronic obstructive pulmonary disease (
COPD) was the 3rd leading cause of death in 2012 worldwide. It is particularly severe in the elderly, who are at risk of death by coughing, mucous hypersecretion, and finally
breathlessness. Recently, anti-
COPD drug development has increased, and many animal screening systems have been studied. Tobacco
smoke animal models are the best known animal screening system, but have several preparation requirements, such as a tobacco
smoke generator and a separate facility to prevent
smoke release. Accordingly, we evaluated the properties of a
lipopolysaccharide (LPS) murine model for
COPD screening and the effect of the time elapsed from 0 to 72 hr after LPS intranasal instillation on various
biomarkers of
COPD severity, such as WBC and neutrophils in bronchoalveolar fluid (BALF),
IgE in serum, histopathology in the lung, and
cytokines (IL-8, TNF-α, IFN-γ, and TGF-β) and
chemokines (CCL-2, CXCL1, CXCL9, CXCL10, and CXCL11) in the respiratory system. Although from 48 hr after LPS treatment several factors which could be evaluated as
biomarkers for
COPD establishment such as WBC and neutrophil in BALF,
IgE in serum,
cytokines (IL-8, TNF-α, and IFN-γ), and
chemokines (CCL-2, CXCL1, CXCL9, CXCL10, and CXCL11) increased at 72 hr the increment of important factors for
COPD establishment such as
IgE,
fibrosis in the lung, and
cytokines (IL-8, TNF-α, and IFN-γ) was more clear. Based on our results, we concluded that the optimal time after LPS intranasal instillation is 72 hr.