Abstract | PURPOSE: METHODS: We used immuno-proteomic methods to survey novel autoantigens expressed in the orbit fat tissue of GO patients and confirmed by enzyme-linked immunosorbent assay (ELISA). RESULTS: One protein spot ( aldehyde dehydrogenase 2 (ALDH2)) revealed high reactivity with the GO serum than did the healthy control serum and was further verified by ELISA. We found that the plasma anti-ALDH2 antibody level was increased in GO patients compared to healthy control donors. In addition, anti-ALDH2 antibody level was correlated with GO activity classified by clinical activity score(r = 0.588, p < 0.001, using Pearson's correlation). CONCLUSIONS: These increased levels of anti-ALDH2 antibody in GO serum suggested that ALDH2 could attribute target autoantigen in GO, and anti-ALDH2 autoantibody might serve as a biomarker for GO and help to predict disease activity.
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Authors | Kai-Chun Cheng, Yu-Jen Wu, Kai-Hung Cheng, Kai-Yuan Cheng, Kuo-Jen Chen, Wen-Chuan Wu, Po-Yen Lee, Cheng-Hsien Chang |
Journal | Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
(Graefes Arch Clin Exp Ophthalmol)
Vol. 256
Issue 6
Pg. 1195-1201
(Jun 2018)
ISSN: 1435-702X [Electronic] Germany |
PMID | 29397435
(Publication Type: Journal Article)
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Chemical References |
- Autoantibodies
- Biomarkers
- ALDH2 protein, human
- Aldehyde Dehydrogenase, Mitochondrial
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Topics |
- Adult
- Aged
- Aldehyde Dehydrogenase, Mitochondrial
(blood, immunology)
- Autoantibodies
(blood)
- Biomarkers
(blood)
- Disease Progression
- Enzyme-Linked Immunosorbent Assay
- Female
- Graves Ophthalmopathy
(blood, immunology)
- Humans
- Male
- Middle Aged
- Proteomics
(methods)
- Young Adult
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