Abstract | BACKGROUND: METHODS: A total of 80 subjects were enrolled in the present study, including 37 patients with MS (relapsing-remitting MS [RRMS; n=18] and secondary progressive MS [SPMS; n=19]), 10 patients with NMOSD and 10 patients with NPSLE as well as 23 healthy subjects. Serum expression levels of three selected miRNAs (miR-145, miR-223 and miR-326) were measured using quantitative real-time polymerase chain reaction (qRT-PCR). Whole blood expression levels of cellular immune response-relevant target genes, including signaling mother against decapentaplegic peptide 3 (SMAD3) and specificity protein 1 (SP1), were also measured using qRT-PCR. RESULTS: In comparison to healthy subjects, only miR-145 and miR-223 were significantly up-regulated in MS patients, whereas, all the analyzed miRNAs revealed insignificant upregulation in NMOSD patients. All the examined miRNAs were significantly down-regulated in NPSLE patients compared to healthy subjects. miR-145, miR-223 and miR-326 expression profile is a promising diagnostic biomarker for MS and NPSLE, but not for NMOSD. This expression profile is capable of differentiating not only among MS, NMOSD and NPSLE, but also between RRMS and SPMS. CONCLUSION:
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Authors | Wessam E Sharaf-Eldin, Nirmeen A Kishk, Yehia Z Gad, Heba Hassan, Mohamed A M Ali, Maha S Zaki, Mohamed R Mohamed, Mona L Essawi |
Journal | Journal of the neurological sciences
(J Neurol Sci)
Vol. 383
Pg. 188-198
(Dec 15 2017)
ISSN: 1878-5883 [Electronic] Netherlands |
PMID | 29246612
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers
- MIRN145 microRNA, human
- MIRN223 microRNA, human
- MIRN326 microRNA, human
- MicroRNAs
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Topics |
- Biomarkers
(blood)
- Cohort Studies
- Diagnosis, Differential
- Disease Progression
- Extracellular Space
(metabolism)
- Gene Expression
- Humans
- MicroRNAs
(blood)
- Multiple Sclerosis, Chronic Progressive
(blood, immunology, therapy)
- Multiple Sclerosis, Relapsing-Remitting
(blood, immunology, therapy)
- Multivariate Analysis
- Neuromyelitis Optica
(blood, immunology, therapy)
- Sensitivity and Specificity
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