The gastroprotective potential of the methanolic extracts from peels (MEPe), seeds (MESe) and pulp (MEPu) of Chrysophyllum cainito L. (Sapotaceae) fruits was evaluated in mice using
ethanol/HCl- and
indomethacin-induced
ulcer, as well as the antiulcer effect of the juice and flour from this fruit. The lowest oral gastroprotective dose of MEPe, MESe and MEPu against
ethanol/HCl was 3, 3 and 10 mg/kg, respectively. Moreover, all extracts increased
mucin secretion at 176, 198 and 193%. Intraperitoneal administration of MEPe (0.3 mg/kg), MESe (0.3 mg/kg) and MEPu (1 mg/kg) also promoted gastroprotection against
ethanol/HCl. In addition, MEPe (3 mg/kg, p.o), MESe (3 mg/kg, p.o) and MEPu (10 mg/kg, p.o) reduced
indomethacin-induced
gastric ulcer in mice by 78, 70 and 50%, respectively. Regarding the mode of action, the gastroprotective effect of MEPe was decreased by the pre-administration of
N-ethylmaleimide (NEM, a sulfhydryl group
chelator, 10 mg/kg, i.p),
glibenclamide (a
potassium channel blocker, 10 mg/kg, i.p),
yohimbine (10 mg/kg, i.p, an
alpha-adrenergic receptor antagonist, 10 mg/kg, i.p) and
indomethacin (a
cyclooxygenase inhibitor, 10 mg/kg, i.p). The gastroprotective effect of MESe was reduced by the pre-administration of NEM,
glibenclamide, N-Nitro-
L-arginine methyl ester (
L-NAME, a
nitric oxide synthase inhibitor, 70 mg/kg, i.p) and
yohimbine, while MEPu had the gastroprotective effect decreased in animals pretreated with NEM and
L-NAME. However, the extracts did not reduce gastric acid secretion. The supplementation with the flour from C. cainito fruit
at 10% by 7 days, but not the juice intake, displayed gastroprotective potential, evidencing the fruit as a promising functional food. Together, the antiulcer effect of extracts of the C. cainito fruit in different experimental models was confirmed by the favoring of mucosal protective mechanisms among different, but complementary, modes of action. In parallel, the gastroprotective effects of the flour from C. cainito fruit were also described.