Abstract | PURPOSE: METHODS: NOD.H-2h4 mice, which are a model of human AIT, were randomly divided into a normal water control group and a high- iodine group. Mice in the high- iodine group were administered 0.05% NaI (~1000 times the normal daily iodine intake), and mice in the control group received sterile water. Furthermore, we evaluated small interfering RNA ( siRNA) interference in spleen mononuclear cell experiments in vitro. RESULTS: In this study, we found that Th17 cells were significantly increased with a high expression of miR-326 in an iodine-induced thyroiditis NOD.H-2h4 mouse model. In addition, the expression of Ets-1 protein, a negative regulator of Th17 differentiation, was significantly decreased. Intriguingly, our analysis showed that Ets-1 protein expression was negatively correlated with miR-326 levels in AIT mice (r = -0.814, p < 0.01). Our study indicated that miR-326 inhibited Ets-1 protein expression and promoted the differentiation of Th17 cells during the onset and development of AIT. The addition of a miR-326 inhibitor reversed Th17 cell production and Ets-1 protein expression, supporting this hypothesis. CONCLUSIONS:
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Authors | Na Zhao, Hongjin Zou, Jing Qin, Chenling Fan, Yongping Liu, Shuo Wang, Zhongyan Shan, Weiping Teng, Yushu Li |
Journal | Endocrine
(Endocrine)
Vol. 59
Issue 1
Pg. 120-129
(01 2018)
ISSN: 1559-0100 [Electronic] United States |
PMID | 29181619
(Publication Type: Journal Article)
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Chemical References |
- Ets1 protein, mouse
- MIRN326 microRNA, mouse
- MicroRNAs
- Proto-Oncogene Protein c-ets-1
- Iodine
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Topics |
- Animals
- Disease Models, Animal
- Female
- Gene Expression Regulation
- Iodine
- Mice
- Mice, Inbred NOD
- Mice, Transgenic
- MicroRNAs
(genetics, physiology)
- Proto-Oncogene Protein c-ets-1
(genetics)
- T-Lymphocytes, Regulatory
(immunology, metabolism)
- Th17 Cells
(immunology, metabolism)
- Thyroiditis, Autoimmune
(chemically induced, genetics, immunology, metabolism)
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