Insulin receptor (IR) insufficiency in β-cells leads to impaired insulin secretion and reduced β-cell
hyperplasia in response to
hyperglycemia. Selective IR deficiency in β-cells in later embryological development may lead to compensatory β-cell
hyperplasia. Although these findings suggest
insulin signaling on the β-cell is important for β-cell function, they are confounded by loss of signaling by the insulinlike
growth factors through the IR. To determine whether
insulin itself is necessary for β-cell development and maturation, we performed a characterization of pancreatic islets in mice with deletions of both nonallelic
insulin genes (Ins1-/-Ins2-/-). We immunostained neonatal Ins1-/-Ins2-/- and Ins1+/+Ins2+/+ pancreata and performed quantitative polymerase chain reaction on isolated neonatal islets.
Insulin-deficient islets had reduced expression of factors normally expressed in maturing β-cells, including muscoloaponeurotic
fibrosarcoma oncogene homolog A, homeodomain
transcription factor 6.1, and
glucose transporter 2. Ins1-/-Ins2-/-β-cells expressed progenitor factors associated with stem cells or dedifferentiated β-cells, including v-myc avian myolocytomatosis viral oncogene lung
carcinoma derived and
homeobox protein NANOG. We replaced
insulin by injection or
islet transplantation to keep mice alive into adulthood to determine whether
insulin replacement was sufficient for the completed maturation of
insulin-deficient β-cells. Short-term
insulin glargine (Lantus®)
injections partially rescued the β-cell phenotype, whereas long-term replacement of
insulin by isogenic
islet transplantation supported the formation of more mature β-cells. Our findings suggest that tightly regulated glycemia,
insulin species, or other islet factors are necessary for β-cell maturation.