Myo-inositol reduces β-catenin activation in colitis.

Abstract

AIM:

To assess dietary myo-inositol in reducing stem cell activation in colitis, and validate pβ-cateninS552 as a biomarker of recurrent dysplasia.

METHODS:

We examined the effects of dietary myo-inositol treatment on inflammation, pβ-cateninS552 and pAkt levels by histology and western blot in IL-10-/- and dextran sodium sulfate-treated colitic mice. Additionally, we assessed nuclear pβ-cateninS552 in patients treated with myo-inositol in a clinical trial, and in patients with and without a history of colitis-induced dysplasia.

RESULTS:

In mice, pβ-cateninS552 staining faithfully reported the effects of myo-inositol in reducing inflammation and intestinal stem cell activation. In a pilot clinical trial of myo-inositol administration in patients with a history of low grade dysplasia (LGD), two patients had reduced numbers of intestinal stem cell activation compared to the placebo control patient. In humans, pβ-cateninS552 staining discriminated ulcerative colitis patients with a history of LGD from those with benign disease.

CONCLUSION:

Enumerating crypts with increased numbers of pβ-cateninS552 - positive cells can be utilized as a biomarker in colitis-associated cancer chemoprevention trials.

• Authors: Emily M Bradford, Corey A Thompson, Tatiana Goretsky, Guang-Yu Yang, Luz M Rodriguez, Linheng Li, Terrence A Barrett
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 23 Issue 28 Pg. 5115-5126 (Jul 28 2017) ISSN: 2219-2840 [Electronic] United States
• PMID: 28811707 (Publication Type: Clinical Trial, Journal Article)

Chemical References

• Biomarkers, Tumor
• CTNNB1 protein, human
• CTNNB1 protein, mouse
• IL10 protein, mouse
• Placebos
• beta Catenin
• Interleukin-10
• Inositol
• Dextran Sulfate

Topics

• Animals
• Biomarkers, Tumor (analysis, metabolism)
• Biopsy
• Cell Nucleus (metabolism)
• Cell Proliferation
• Colitis, Ulcerative (chemically induced, drug therapy, pathology)
• Colon (cytology, drug effects, pathology)
• Colorectal Neoplasms (diagnosis, prevention & control)
• Dextran Sulfate (toxicity)
• Disease Models, Animal
• Humans
• Immunohistochemistry
• Inositol (pharmacology, therapeutic use)
• Interleukin-10 (genetics, metabolism)
• Mice
• Mice, Knockout
• Phosphorylation
• Pilot Projects
• Placebos
• Signal Transduction
• Stem Cells (drug effects)
• beta Catenin (analysis, metabolism)