Sophora subprosrate polysaccharide inhibited cytokine/chemokine secretion via suppression of histone acetylation modification and NF-κb activation in PCV2 infected swine alveolar macrophage.

Abstract

In the present study, effect of Sophora subprosrate polysaccharide on PCV2 infection-induced inflammation and histone acetylation modification in swine alveolar macrophage 3D4/2 cells was described for the first time. The relationship between histone acetylation modifications and inflammation response was investigated. The results showed that PCV2 infection induced inflammation by promoting the secretion of TNF-α, IL-1β, IL-6 and IL-10 in 3D4/2 cells. The production of TNF-α, IL-1β and IL-6 and their mRNA expression levels markedly decreased while the level and mRNA expression of IL-10 were elevated when the cells were treated with Sophora subprosrate polysaccharide. The SSP also decreased the activity of HATs, histone H3 acetylation (Ac-H3) and histone H4 acetylation (Ac-H4), p65 phosphorylation (P-p65) in the cells infected with PCV2 while HDACs activity was down-regulated, which involved in the inhibitory effect of SSP on histone acetylation and NF-κB signaling pathways activation. Down-regulation of HAT1 mRNA expression and up-regulation of HDAC1 mRNA expression further support the inhibitory effect of SSP on histone acetylation. In conclusion, Sophora subprosrate polysaccharide antagonized inflammatory responses induced by PCV2, via mechanisms involved in histone acetylation and NF-κB signaling pathways.

• Authors: Jian Yang, Hong-Lian Tan, Li-Yuan Gu, Man-Ling Song, Yuan-Ying Wu, Jian-Bo Peng, Zong-Bao Lan, Ying-Yi Wei, Ting-Jun Hu
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 104 Issue Pt A Pg. 900-908 (Nov 2017) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 28666830 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Chemokines
• NF-kappa B
• Polysaccharides
• RNA, Messenger
• Histone Acetyltransferases
• Histone Deacetylases

Topics

• Acetylation (drug effects)
• Animals
• Cell Line
• Cell Survival (drug effects)
• Chemokines (metabolism)
• Circovirus (physiology)
• Gene Expression Regulation (drug effects)
• Histone Acetyltransferases (metabolism)
• Histone Deacetylases (metabolism)
• Macrophages, Alveolar (cytology, drug effects, metabolism, virology)
• NF-kappa B (metabolism)
• Polysaccharides (pharmacology)
• RNA, Messenger (genetics, metabolism)
• Sophora (chemistry)
• Swine