Schisandrin B attenuates the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury via inhibition of p53 signaling in adult rats.

Abstract

Schisandrin B is an active monomer of the Chinese magnolia vine (Schisandra chinensis) that can reduce transaminase activity in liver cells, inhibit lipid peroxidation, enhance antioxidant status, has protective effects in the liver and has antitumor effects. The present study investigated the potential protective effects of schisandrin B on the p53 signaling pathway in attenuating the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury (TSCI) in adult rats. Behavioral examination, inclined plate test and spinal cord water content were used to evaluate the protective effect of schisandrin B in TSCI rats. The expression levels of superoxide dismutase (SOD), malondialdehyde (MDA), nuclear factor (NF)‑κB subunit p65 and tumor necrosis factor (TNF)‑α were examined using ELISA kits. Western blot analysis was performed to analyze the protein expression of caspase‑3 and phosphorylated (p)‑p53 in TSCI rats. In the present study, schisandrin B improved behavioral examination results and the maximum angle of inclined plate test, and inhibited spinal cord water content in rats with TSCI. Notably, schisandrin B reduced the activation of traumatic injury‑associated pathways, including SOD, MDA, NF‑κB p65 and TNF‑α, in TSCI rats. In addition, schisandrin B suppressed the TSCI‑induced expression of caspase‑3 and p‑p53 in TSCI rats. These results indicated that schisandrin B may attenuate the inflammatory response, oxidative stress and apoptosis in TSCI rats by inhibiting the p53 signaling pathway in adult rats.

• Authors: D Q Xin, Z M Hu, H J Huo, X J Yang, D Han, W H Xing, Y Zhao, Q H Qiu
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 16 Issue 1 Pg. 533-538 (Jul 2017) ISSN: 1791-3004 [Electronic] Greece
• PMID: 28560444 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Biomarkers
• Cyclooctanes
• Lignans
• NF-kappa B
• Polycyclic Compounds
• Tumor Necrosis Factor-alpha
• Tumor Suppressor Protein p53
• schizandrin B
• Caspase 3

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology)
• Apoptosis (drug effects)
• Biomarkers
• Caspase 3 (metabolism)
• Cyclooctanes (chemistry, pharmacology)
• Disease Models, Animal
• Lignans (chemistry, pharmacology)
• Male
• NF-kappa B (metabolism)
• Oxidative Stress (drug effects)
• Polycyclic Compounds (chemistry, pharmacology)
• Rats
• Signal Transduction (drug effects)
• Spinal Cord Injuries (drug therapy, metabolism, pathology)
• Tumor Necrosis Factor-alpha (metabolism)
• Tumor Suppressor Protein p53 (metabolism)