Abstract | BACKGROUND:
Galla Rhois has been considered to have medicinal properties against diarrhea, excessive sweating, bleeding, and chronic cough in Asian countries. Gallotannins, which are Galla Rhois-derived tannins, have been reported to possess biological and pharmacological activities, especially anticancer activity. In this study, we evaluated the effect of steaming at a temperature over 120 °C on the chemical constituents and biological activities of the water extract of Galla Rhois (GRE). METHODS: GRE was steamed at a temperature over 120 °C (AGRE), and its specific constituents were analyzed; the results were validated using a high-performance liquid chromatography-diode array detector system. To evaluate the anticancer effect of GRE and AGRE, cell viability assay, cell cycle analysis, and Western blot analysis were performed in HCT116 human colon cancer cells. RESULTS: CONCLUSION: Compared with GRE, AGRE is more potent in its ability to induce apoptosis in HCT116 cells; therefore, we suggest that the steaming process may be useful as a feasible method for improving the anticancer effect of GRE.
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Authors | Nam-Hui Yim, Min Jung Gu, Youn-Hwan Hwang, Won-Kyung Cho, Jin Yeul Ma |
Journal | Integrative medicine research
(Integr Med Res)
Vol. 5
Issue 4
Pg. 284-292
(Dec 2016)
ISSN: 2213-4220 [Print] Netherlands |
PMID | 28462130
(Publication Type: Journal Article)
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