Flavonoids from Potentilla parvifolia Fisch. and Their Neuroprotective Effects in Human Neuroblastoma SH-SY5Y Cells in vitro.

Abstract

Potentilla parvifolia Fisch. (Rosaceae) is a traditional medicinal plant in P. R. China. In this study, seven flavonoids, ayanin (1), tricin (2), quercetin (3), tiliroside (4), miquelianin (5), isoquercitrin (6), and astragalin (7), were separated and purified from ethyl acetate extractive fractions from ethanol extracts of P. parvifolia using a combination of sevaral chromatographic methods. The human neuroblastoma SH-SY5Y cells were differentiated with all trans-retinoic acid and treated with okadaic acid to induce tau protein phosphorylation and synaptic atrophy, which could establish an Alzheimer's disease cell model. The neuroprotective effects of these flavonoids in cellular were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3 and 4 had the better neuroprotective effects.

• Authors: Zhenzhen Yuan, Guangxiang Luan, Zhenhua Wang, Xueyan Hao, Ji Li, Yourui Suo, Gang Li, Honglun Wang
• Journal: Chemistry & biodiversity (Chem Biodivers) Vol. 14 Issue 6 (Jun 2017) ISSN: 1612-1880 [Electronic] Switzerland
• PMID: 28294523 (Publication Type: Journal Article)
• Copyright: © 2017 Wiley-VHCA AG, Zurich, Switzerland.

Chemical References

• Flavonoids
• Neuroprotective Agents
• Plant Extracts
• tau Proteins

Topics

• Alzheimer Disease (chemically induced, drug therapy, pathology)
• Cell Differentiation
• Cell Line, Tumor
• Flavonoids (isolation & purification, pharmacology)
• Humans
• Neuroblastoma (drug therapy, pathology)
• Neuroprotective Agents (isolation & purification, pharmacology)
• Phosphorylation
• Plant Extracts (chemistry)
• Potentilla (chemistry)
• tau Proteins (metabolism)