Antihyperlipidaemic effect of triterpenic acid-enriched fraction from Cyclocarya paliurus leaves in hyperlipidaemic rats.

Abstract

CONTEXT:

Cyclocarya paliurus (Batal) Iljinskaja (Juglandaceae) is an edible and medicinal plant; the leaves are used in Chinese folkloric medicine to treat dyslipidaemia and diabetes.

OBJECTIVE:

This study evaluates the antihyperlipidaemic potential of the triterpenic acid-enriched fraction (TAE) from C. paliurus and the underlying mechanism.

MATERIALS AND METHODS:

The hyperlipidaemic rats were induced by high fat diet for 6 weeks. After oral administration of TAE (200 and 400 mg/kg), the neutral fraction (150 and 300 mg/kg) and statin (4 mg/kg) to the hyperlipidaemic rats for 4 weeks, lipid profile and apolipoprotein (apoB48) level in plasma, and the expression levels of apoB48, microsomal triglyceride transfer protein (MTP), phosphorylation of mitogen-activated protein kinase (MAPK) and tumour necrosis factor α (TNF-α) in intestine were examined. The main constituents in the TAE were identified by HPLC-MS.

RESULTS:

TAE administration (400 mg/kg) decreased the levels of atherogenic lipids in serum and liver (p < 0.05) and increased serum high-density lipoprotein cholesterol by 19.7%. Furthermore, TAE treatment (200 and 400 mg/kg) decreased plasma apoB48 level by 15.3 and 19.5%, downregulated intestinal apoB48 and MTP expression levels (p < 0.05), and inhibited TNF-α expression by 36.2 and 56.2% and the phosphorylation level of MAPK by 8.8 and 13.2%, respectively. HPLC analysis revealed the presence of pentacyclic- and tetracyclic-triterpene acids in TAE.

CONCLUSION AND DISCUSSION:

These findings suggested that TAE possessed antihyperlipidaemic activity partially involved in the inhibitory effect on apoB48 overproduction, which may provide evidence about its potential role in ameliorating dyslipidaemia.

• Authors: Zhengfeng Wu, Tianhong Gao, Rongling Zhong, Zi Lin, Cuihua Jiang, Sheng Ouyang, Ming Zhao, Chuntao Che, Jian Zhang, Zhiqi Yin
• Journal: Pharmaceutical biology (Pharm Biol) Vol. 55 Issue 1 Pg. 712-721 (Dec 2017) ISSN: 1744-5116 [Electronic] England
• PMID: 28140736 (Publication Type: Journal Article)

Chemical References

• Apolipoprotein B-48
• Hypolipidemic Agents
• Lipids
• Plant Extracts
• Triterpenes
• Tumor Necrosis Factor-alpha
• Mitogen-Activated Protein Kinases

Topics

• Animals
• Apolipoprotein B-48 (antagonists & inhibitors, blood)
• Hypolipidemic Agents (pharmacology)
• Juglandaceae (chemistry)
• Lipids (blood)
• Male
• Mitogen-Activated Protein Kinases (metabolism)
• Plant Extracts (pharmacology)
• Plant Leaves (chemistry)
• Rats
• Rats, Sprague-Dawley
• Triterpenes (pharmacology)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors)