MiR-320a effectively suppresses lung adenocarcinoma cell proliferation and metastasis by regulating STAT3 signals.

Abstract

MicroRNAs play important roles in tumorigenesis of various types of cancers. MiR-320a can inhibits cell proliferation of some cancers, but the biologic roles of miR-320a in lung cancer need to be further studied. Here, we investigated the roles of miR-320a in suppressing the proliferation of lung adenocarcinoma cells. MiR-320a treatment was found to effectively suppress LTEP-a-2 and A549 cell proliferation, and induce more apoptotic cells with irradiation treatment compared with control treatment. Our results also showed that miR-320a, as a novel miRNA, directly regulated signal transducer and activator of transcription 3 (STAT3) and its signals, such as Bcl-2, Bax, and Caspase 3. The siRNA-inhibited STAT3 levels further proved its roles in regulating STAT3 signals. Moreover, miR-320a treatment effectively suppressed cancer cell growth in mice xenografts compared with controls, and significantly inhibited cell migration in vitro and in vivo. Our findings collectively demonstrated that miR-320a, by directly regulating STAT3 signals, not only suppressed cell proliferation and metastasis, but also enhanced irradiation-induced apoptosis of adenocarcinomia cells.

• Authors: Qing Lv, Jin-Xia Hu, You-Jie Li, Ning Xie, Dan Dan Song, Wei Zhao, Yun-Fei Yan, Bao-Sheng Li, Ping-Yu Wang, Shu-Yang Xie
• Journal: Cancer biology & therapy (Cancer Biol Ther) Vol. 18 Issue 3 Pg. 142-151 (03 04 2017) ISSN: 1555-8576 [Electronic] United States
• PMID: 28106481 (Publication Type: Journal Article)

Chemical References

• MIRN320 microRNA, human
• MicroRNAs
• STAT3 Transcription Factor
• STAT3 protein, human

Topics

• A549 Cells
• Adenocarcinoma (genetics, pathology, radiotherapy, therapy)
• Adenocarcinoma of Lung
• Animals
• Cell Proliferation (genetics)
• Humans
• Lung Neoplasms (genetics, pathology, radiotherapy, therapy)
• Male
• Mice
• Mice, Inbred BALB C
• MicroRNAs (administration & dosage, genetics)
• Neoplasm Metastasis
• STAT3 Transcription Factor (antagonists & inhibitors, genetics, metabolism)
• Signal Transduction
• Transfection
• Xenograft Model Antitumor Assays