Obesity/insulin resistance rather than liver fat increases coagulation factor activities and expression in humans.

Abstract

Increased liver fat may be caused by insulin resistance and adipose tissue inflammation or by the common I148M variant in PNPLA3 at rs738409, which lacks both of these features. We hypothesised that obesity/insulin resistance rather than liver fat increases circulating coagulation factor activities. We measured plasma prothrombin time (PT, Owren method), activated partial thromboplastin time (APTT), activities of several coagulation factors, VWF:RCo and fibrinogen, and D-dimer concentration in 92 subjects divided into groups based on insulin sensitivity [insulin-resistant ('IR') versus insulin-sensitive ('IS')] and PNPLA3 genotype (PNPLA3148MM/MI vs PNPLA3148II). Liver fat content (1H-MRS) was similarly increased in 'IR' (13 ± 1 %) and PNPLA3148MM/MI (12 ± 2 %) as compared to 'IS' (6 ± 1 %, p<0.05) and PNPLA3148II (8 ± 1 %, p<0.05), respectively. FVIII, FIX, FXIII, fibrinogen and VWF:RCo activities were increased, and PT and APTT shortened in 'IR' versus 'IS', in contrast to these factors being similar between PNPLA3148MM/MI and PNPLA3148II groups. In subjects undergoing a liver biopsy and entirely lacking the I148M variant, insulin-resistant subjects had higher hepatic expression of F8, F9 and FGG than equally obese insulin-sensitive subjects. Expression of pro-inflammatory genes in adipose tissue correlated positively with PT (% of normal), circulating FVIII, FIX, FXI, VWR:RCo and fibrinogen, and expression of anti-inflammatory genes negatively with PT (%), FIX and fibrinogen. We conclude that obesity/insulin resistance rather than an increase in liver fat is associated with a procoagulant plasma profile. This reflects adipose tissue inflammation and increased hepatic production of coagulation factors and their susceptibility for activation.

• Authors: Susanna Lallukka, Panu K Luukkonen, You Zhou, Elina Isokuortti, Marja Leivonen, Anne Juuti, Antti Hakkarainen, Marju Orho-Melander, Nina Lundbom, Vesa M Olkkonen, Riitta Lassila, Hannele Yki-Järvinen
• Journal: Thrombosis and haemostasis (Thromb Haemost) Vol. 117 Issue 2 Pg. 286-294 (01 26 2017) ISSN: 2567-689X [Electronic] Germany
• PMID: 27929200 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Blood Coagulation Factors
• Cytokines
• Inflammation Mediators
• Membrane Proteins
• Lipase
• adiponutrin, human

Topics

• Adipose Tissue (metabolism, pathology)
• Adiposity
• Adult
• Biomarkers (blood)
• Biopsy
• Blood Coagulation
• Blood Coagulation Factors (genetics, metabolism)
• Cytokines (blood)
• Female
• Genetic Predisposition to Disease
• Humans
• Inflammation Mediators (blood)
• Insulin Resistance
• Lipase (genetics)
• Liver (metabolism, pathology)
• Male
• Membrane Proteins (genetics)
• Middle Aged
• Non-alcoholic Fatty Liver Disease (blood, diagnosis, genetics)
• Obesity (blood, diagnosis)
• Partial Thromboplastin Time
• Phenotype
• Polymorphism, Single Nucleotide
• Prothrombin Time
• Up-Regulation