Food-advanced glycation end products aggravate the diabetic vascular complications via modulating the AGEs/RAGE pathway.

Abstract

The aim of this study was to investigate the effects of high-advanced glycation end products (AGEs) diet on diabetic vascular complications. The Streptozocin (STZ)-induced diabetic mice were fed with high-AGEs diet. Diabetic characteristics, indicators of renal and cardiovascular functions, and pathohistology of pancreas, heart and renal were evaluated. AGEs/RAGE/ROS pathway parameters were determined. During the experiments, the diabetic mice exhibited typical characteristics including weight loss, polydipsia, polyphagia, polyuria, high-blood glucose, and low-serum insulin levels. However, high-AGEs diet effectively aggravated these diabetic characteristics. It also increased the 24-h urine protein levels, serum levels of urea nitrogen, creatinine, c-reactive protein (CRP), low density lipoprotein (LDL), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in the diabetic mice. High-AGEs diet deteriorated the histology of pancreas, heart, and kidneys, and caused structural alterations of endothelial cells, mesangial cells and podocytes in renal cortex. Eventually, high-AGEs diet contributed to the high-AGE levels in serum and kidneys, high-levels of reactive oxygen species (ROS) and low-levels of superoxide dismutase (SOD) in serum, heart, and kidneys. It also upregulated RAGE mRNA and protein expression in heart and kidneys. Our results showed that high-AGEs diet deteriorated vascular complications in the diabetic mice. The activation of AGEs/RAGE/ROS pathway may be involved in the pathogenesis of vascular complications in diabetes.

• Authors: Xing Lv, Gao-Hong Lv, Guo-Ying Dai, Hong-Mei Sun, Hui-Qin Xu
• Journal: Chinese journal of natural medicines (Chin J Nat Med) Vol. 14 Issue 11 Pg. 844-855 (Nov 2016) ISSN: 1875-5364 [Electronic] China
• PMID: 27914528 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

Chemical References

• Glycation End Products, Advanced
• Interleukin-6
• Reactive Oxygen Species
• Receptor for Advanced Glycation End Products
• Tumor Necrosis Factor-alpha
• Superoxide Dismutase

Topics

• Animals
• Diabetes Mellitus, Experimental (complications, metabolism)
• Diabetic Angiopathies (genetics, metabolism)
• Diet (adverse effects)
• Glycation End Products, Advanced (metabolism)
• Humans
• Interleukin-6 (metabolism)
• Kidney (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Oxidative Stress
• Pancreas (metabolism)
• Reactive Oxygen Species (metabolism)
• Receptor for Advanced Glycation End Products (genetics, metabolism)
• Superoxide Dismutase (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)