MicroRNA-98 plays a critical role in experimental myocarditis.

Abstract

BACKGROUND AND AIMS:

Myocarditis is inflammation in the heart; its pathogenesis is to be further investigated. Activities of micro RNAs (miR) are associated with immune inflammation. This study tests a hypothesis that miR-98 is involved in the development of myocarditis.

METHODS:

BALB/c mice were immunized with cardiac α-myosin heavy chain peptides (MyHC-α) to induce myocarditis. The effects of miR-98 on regulation of interleukin (IL)-10 were assessed by real time RT-PCR.

RESULTS:

Mice immunized with MyHC-α showed myocarditis and lower frequency of IL-10+ B cells (B10 cell) in the hearts. Expression of miR-98 was higher, IL-10 was lower, in B cells isolated from the mouse hearts with myocarditis, which was negatively correlated with each other. Exposure to tumor necrosis factor-α up regulated miR-98 expression in B cells. Over-expression of miR-98 suppressed IL-10 expression in B cells. Blocking miR-98 or adoptively transplanting B10 cells attenuated experimental myocarditis.

CONCLUSIONS:

miR-98 suppresses IL-10 expression in B cells in the heart, which plays an important role in myocarditis. MiR-98 may be a therapeutic target in the treatment of myocarditis.

• Authors: Xiao Chen, Shuo Dong, Ningning Zhang, Liang Chen, Mao-Gang Li, Ping-Chang Yang, Jiangping Song
• Journal: International journal of cardiology (Int J Cardiol) Vol. 229 Pg. 75-81 (Feb 15 2017) ISSN: 1874-1754 [Electronic] Netherlands
• PMID: 27913008 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• MIRN98 microRNA, human
• MicroRNAs
• Interleukin-10

Topics

• Adult
• Animals
• B-Lymphocytes (immunology, metabolism)
• Cells, Cultured
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Female
• Flow Cytometry
• Gene Expression Regulation
• Humans
• Immunity, Cellular
• Interleukin-10 (biosynthesis, genetics)
• Male
• Mice
• Mice, Inbred BALB C
• MicroRNAs (biosynthesis, genetics)
• Middle Aged
• Myocarditis (genetics, immunology, metabolism)
• Myocardium (metabolism, pathology)
• Real-Time Polymerase Chain Reaction