Elevated IL-17A and IL-22 regulate expression of inducible CD38 and Zap-70 in chronic lymphocytic leukemia.

Abstract

BACKGROUND:

In this study, we investigated the role and expression of interleukin (IL)-17A and IL-22 in chronic lymphocytic leukemia.

METHODS:

We evaluated the expression of markers above on CLL by ELISA, qRT-PCR, flow cytometric analysis and nonparametric Kruskal-Wallis test.

RESULTS:

Quantitative RT-PCR revealed that the mRNA levels of IL-17A and IL-22 in PBMCs of CLL patients were upregulated compared with those from healthy subjects (mean ± SD: 1.96 ± 0.232 vs.0.72 ± 0.15, P < 0.001 and mean ± SD: 2.45 ± 0.534 vs.0.81 ± 0.26, P < 0.001, respectivily). In addition, findings showed that the IL-17A and IL-22 plasma level was significantly elevated than that from healthy control group (P  < 0.001). The median IL-17A and IL-22 in CLL patients and healthy control group were 48.28 ± 17.2 pg mL-1 ; 20.01 ± 11.16 pg mL-1 and 58.68 ± 23.4 pg mL-1 ;16.47 ± 10.31 P < 0.001, respectively. The levels of IL-17A and IL-22 was not significantly associated with the different stages of disease (Rai stages; Kruskal-Wallis test P > 0.05).No significant relationship was found between expression of CD38 and higher median serum levels of IL-17A in patients, but patients with negative expression of ZAP-70 showed a significant association with higher median serum levels of IL-17A compared with healthy subjects. (57.84 pg mL-1 vs. 31.67 pg mL-1 ; P = 0.016).

CONCLUSION:

IL-22 is elevated and associated with CD38 and Zap-70 expression in patients with CLL. No significant correlation was found between expression of CD38 and increased levels of IL-17A, negative expression of ZAP-70 showed a significant association with increased levels of IL-17A. © 2016 International Clinical Cytometry Society.

• Authors: Samaneh Kouzegaran, Soheila Siroosbakht, Bahram Fariborz Farsad, Bijan Rezakhaniha, Banafshe Dormanesh, Vahid Behnod, Amir Saber Tanha
• Journal: Cytometry. Part B, Clinical cytometry (Cytometry B Clin Cytom) Vol. 94 Issue 1 Pg. 143-147 (01 2018) ISSN: 1552-4957 [Electronic] United States
• PMID: 27718514 (Publication Type: Journal Article)
• Copyright: © 2016 International Clinical Cytometry Society.

Chemical References

• IL17A protein, human
• Interleukin-17
• Interleukins
• Membrane Glycoproteins
• ZAP-70 Protein-Tyrosine Kinase
• ZAP70 protein, human
• CD38 protein, human
• ADP-ribosyl Cyclase 1

Topics

• ADP-ribosyl Cyclase 1 (metabolism)
• Adult
• Aged
• Female
• Flow Cytometry (methods)
• Humans
• Interleukin-17 (metabolism)
• Interleukins (metabolism)
• Leukemia, Lymphocytic, Chronic, B-Cell (metabolism)
• Male
• Membrane Glycoproteins (metabolism)
• Middle Aged
• Up-Regulation (physiology)
• ZAP-70 Protein-Tyrosine Kinase (metabolism)
• Interleukin-22