Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags.

Abstract

CONTEXT AND OBJECTIVE:

Human hepatocellular carcinoma (HCC) is a severe malignant disease, and accurate and reliable diagnostic markers are still needed. This study was aimed for the discovery of novel marker candidates by quantitative proteomics.

METHODS AND RESULTS:

Proteomic differences between HCC and nontumorous liver tissue were studied by mass spectrometry. Among several significantly upregulated proteins, translocator protein 18 (TSPO) and Ras-related protein Rab-1A (RAB1A) were selected for verification by immunohistochemistry in an independent cohort. For RAB1A, a high accuracy for the discrimination of HCC and nontumorous liver tissue was observed.

CONCLUSION:

RAB1A was verified to be a potent biomarker candidate for HCC.

• Authors: Dominik Andre Megger, Kristin Rosowski, Maike Ahrens, Thilo Bracht, Martin Eisenacher, Jörg F Schlaak, Frank Weber, Andreas-Claudius Hoffmann, Helmut E Meyer, Hideo A Baba, Barbara Sitek
• Journal: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (Biomarkers) Vol. 22 Issue 2 Pg. 113-122 (Mar 2017) ISSN: 1366-5804 [Electronic] England
• PMID: 27467182 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Proteome
• rab1 GTP-Binding Proteins

Topics

• Biomarkers, Tumor (analysis)
• Carcinoma, Hepatocellular (diagnosis)
• Humans
• Liver Neoplasms (diagnosis)
• Proteome (analysis)
• Proteomics (methods)
• Tandem Mass Spectrometry
• Up-Regulation
• rab1 GTP-Binding Proteins (analysis)