miR-483-3p regulates hyperglycaemia-induced cardiomyocyte apoptosis in transgenic mice.

Abstract

Diabetic cardiomyopathy represents severe heart complications, and is the leading cause of morbidity and mortality among patients with diabetes. Although a few microRNAs (miRNAs) have been implicated in diabetes-related complications, a functional association between miRNAs and cardiac dysfunction in diabetic cardiomyopathy remains to be demonstrated. Our results show that miR-483-3p is upregulated in streptozotocin-induced diabetic mice, and cultured cardiomyocytes mimicking hyperglycemia. Overexpressing miR-483-3p in transgenic mice with diabetes mellitus (DM) exacerbated cardiomyocyte apoptosis by transcriptionally repressing insulin growth factor 1 (IGF1). Therefore, we have uncovered a novel signaling pathway, involving miR-483-3p-IGF1, that promotes myocardial cell apoptosis under high blood-glucose condition. Further, our study indicates that miR-483-3p could be a potential therapeutic target for managing diabetes-associated heart complications.

• Authors: Yu Qiao, Yanli Zhao, Yan Liu, Ning Ma, Chuxuan Wang, Jiaqi Zou, Zhiyan Liu, Zhongqiu Zhou, Dong Han, Jun He, Qian Sun, Yicong Liu, Changqing Xu, Zhimin Du, Hui Huang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 477 Issue 4 Pg. 541-547 (09 02 2016) ISSN: 1090-2104 [Electronic] United States
• PMID: 27346130 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• MicroRNAs
• Mirn483 microRNA, mouse
• Streptozocin

Topics

• Animals
• Apoptosis (genetics)
• Cell Line
• Diabetes Mellitus, Experimental (genetics)
• Hyperglycemia (genetics)
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• MicroRNAs (genetics)
• Myocytes, Cardiac (pathology)
• Rats
• Streptozocin