Evaluation of polypropylene mesh coated with biological hydrogels for temporary closure of open abdomen.

Abstract

Polypropylene mesh, as a temporary abdominal closure device, may cause mechanical intestine injury and inflammatory response. Chitosan/gelatin hydrogel has excellent biocompatibility, soft and elastic properties. This work is to assess the effects of the chitosan/gelatin hydrogel coated polypropylene mesh on open abdomen wounds. Histological analysis and detection of healing-related factors were conducted to evaluate the inflammation and wound healing process. After 1-day implantation in a murine model of open abdomen, the coated polypropylene mesh, compared with simple polypropylene mesh, demonstrated well protection of the intestine serosa. After 14-day implantation, it reduced the inflammation response by down-regulating the cytokines interleukin-6 and tumor necrosis factor-α, and up-regulating the anti-inflammatory factor interleukin-10. Meanwhile, the composite stimulated granulation tissue growth, and promoted matrix deposition and angiogenesis after 7 and 14 days. In conclusion, the modified temporary abdominal closure composite could significantly protect the intestines from mechanical damage and accelerate wound healing.

• Authors: Youming Deng, Jianan Ren, Guopu Chen, Guanwei Li, Kun Guo, Qiongyuan Hu, Xiuwen Wu, Gefei Wang, Guosheng Gu, Jieshou Li
• Journal: Journal of biomaterials applications (J Biomater Appl) Vol. 31 Issue 2 Pg. 302-14 (08 2016) ISSN: 1530-8022 [Electronic] England
• PMID: 27114442 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2016.

Chemical References

• Anti-Inflammatory Agents
• Coated Materials, Biocompatible
• Hydrogels
• Interleukin-6
• Polypropylenes
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Gelatin
• Chitosan

Topics

• Abdominal Wound Closure Techniques
• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Chitosan (chemistry)
• Coated Materials, Biocompatible (therapeutic use)
• Endothelial Cells (drug effects)
• Gelatin (chemistry)
• Granulation Tissue (drug effects)
• Hydrogels (therapeutic use)
• Interleukin-10 (metabolism)
• Interleukin-6 (metabolism)
• Male
• Microscopy, Electron, Scanning
• Polypropylenes (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Spectroscopy, Fourier Transform Infrared
• Surgical Mesh
• Tumor Necrosis Factor-alpha (metabolism)
• Wound Healing