Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: Tissue microarrays were used to detect CTSB expression in 280 patients and to examine the association between CTSB expression and clinicopathological parameters. In addition, the metastatic effects of the CTSB knockdown on two oral cancer cell lines were investigated by transwell migration assay. Cytoplasmic CTSB expression was detected in 34.6% (97/280) of patients. CTSB expression was correlated with positive lymph node metastasis (p = 0.007) and higher tumor grade (p = 0.008) but not with tumor size and distant metastasis. In addition, multivariate analysis using a Cox proportional hazards model revealed a higher hazard ratio, demonstrating that CTSB expression was an independent unfavorable prognostic factor in buccal mucosa carcinoma patients. Furthermore, the Kaplan-Meier curve revealed that buccal mucosa OSCC patients with positive CTSB expression had significantly shorter overall survival. Moreover, treatment with the CTSB siRNA exerted an inhibitory effect on migration in OC2 and CAL27 oral cancer cells. CONCLUSIONS: We conclude that CTSB expression may be useful for determining OSCC prognosis, particularly for patients with lymph node metastasis, and may function as a biomarker of the survival of OSCC patients in Taiwan.
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Authors | Wei-En Yang, Chuan-Chen Ho, Shun-Fa Yang, Shu-Hui Lin, Kun-Tu Yeh, Chiao-Wen Lin, Mu-Kuan Chen |
Journal | PloS one
(PLoS One)
Vol. 11
Issue 3
Pg. e0152165
( 2016)
ISSN: 1932-6203 [Electronic] United States |
PMID | 27031837
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Small Interfering
- Cathepsin B
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Topics |
- Adult
- Aged
- Carcinoma, Squamous Cell
(diagnosis, genetics, pathology)
- Cathepsin B
(analysis, genetics)
- Cell Line, Tumor
- Cell Movement
- Disease Progression
- Female
- Humans
- Kaplan-Meier Estimate
- Lymphatic Metastasis
(diagnosis, genetics, pathology)
- Male
- Middle Aged
- Mouth
(pathology)
- Mouth Neoplasms
(diagnosis, genetics, pathology)
- Prognosis
- RNA Interference
- RNA, Small Interfering
(genetics)
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