Abstract |
The development of effective and stable carriers of small interfering RNA ( siRNA) is important for treating cancer with multidrug resistance (MDR). We developed a new gene and drug co-delivery system and checked its characteristics. Low-density lipoprotein ( LDL) was coupled with N-succinyl chitosan (NSC) Lipoic acid (LA) micelles and co-delivered MDR1 siRNA and paclitaxel (PTX- siRNA/ LDL-NSC-LA) to enhance antitumor effects by silencing the MDR gene of tumors (Li et al., Adv Mater 2014;26:8217-8224). In our study, we developed a new type of containing paclitaxel-loaded micelles and siRNA-loaded LDL nanoparticle. This "binary polymer" is pH and reduction dual-sensitive core-crosslinked micelles. PTX- siRNA/ LDL-NSC-LA had an average particle size of (171.6 ± 6.42) nm, entrapment efficiency of (93.92 ± 1.06) %, and drug-loading amount of (12.35% ± 0.87) %. In vitro, MCF-7 cells, high expressed LDL receptor, were more sensitive to this delivery system than to taxol® and cell activity was inhibited significantly. Fluorescence microscopy showed that PTX- siRNA/ LDL-NSC-LA was uptaken very conveniently and played a key role in antitumor activity. PTX- siRNA/ LDL-NSC-LA protected the siRNA from degradation by macrophage phagocytosis and evidently down-regulated the level of mdr1 mRNA as well as the expression of P-gp. We tested the target ability of PTX- siRNA/ LDL-NSC-LA in vivo in tumor-bearing nude mice. Results showed that this system could directly deliver siRNA and PTX to cancer cells. Thus, new co-delivering siRNA and antitumor drugs should be explored for solving MDR in cancer. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1114-1125, 2017.
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Authors | Shu-Di Yang, Wen-Jing Zhu, Qiao-Ling Zhu, Wei-Liang Chen, Zhao-Xiang Ren, Fang Li, Zhi-Qiang Yuan, Ji-Zhao Li, Yang Liu, Xiao-Feng Zhou, Chun Liu, Xue-Nong Zhang |
Journal | Journal of biomedical materials research. Part B, Applied biomaterials
(J Biomed Mater Res B Appl Biomater)
Vol. 105
Issue 5
Pg. 1114-1125
(07 2017)
ISSN: 1552-4981 [Electronic] United States |
PMID | 27008163
(Publication Type: Journal Article)
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Copyright | © 2016 Wiley Periodicals, Inc. |
Chemical References |
- ABCB1 protein, human
- ATP Binding Cassette Transporter, Subfamily B
- Lipoproteins, LDL
- Micelles
- Neoplasm Proteins
- RNA, Small Interfering
- Thioctic Acid
- Chitosan
- Paclitaxel
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Topics |
- ATP Binding Cassette Transporter, Subfamily B
(antagonists & inhibitors, biosynthesis, genetics)
- Animals
- Chitosan
(chemistry, pharmacology)
- Female
- Gene Transfer Techniques
- Humans
- Lipoproteins, LDL
(chemistry, pharmacology)
- MCF-7 Cells
- Mice
- Mice, Nude
- Micelles
- Neoplasm Proteins
(antagonists & inhibitors, biosynthesis, genetics)
- Neoplasms, Experimental
(genetics, metabolism, pathology, therapy)
- Paclitaxel
(pharmacology)
- RNA, Small Interfering
(biosynthesis, genetics)
- Thioctic Acid
(chemistry, pharmacology)
- Xenograft Model Antitumor Assays
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