IL-17 promoted the inhibition of medulloblastoma in mice by splenocyte injection.

Abstract

BACKGROUND:

Interleukin 17 (IL-17) is a proinflammatory cytokine produced by a new subset of activated CD4+ T cells, Th17 cells. We previously showed that increased Th17 cell populations were presented in human medulloblastoma-infiltrating T cells and peripheral blood. In this study, we attempted to address the possible role of Th17 cells in the biologic activity of IL-17 for tumor control.

METHODS:

We grafted fresh surgically obtained medulloblastoma into syngeneic athymic nude/nude mice. We intrapertonially injected splenocyte and murine IL-17 in mice on the second day. The tumor volume and the life spans of the mice were measured. Meanwhile, the IL-17, IL-6, IL-23, Ccl2, Ccl20 and IFN-gamma expression in the tumors was also examined by real-time PCR, Western blot and enzyme-linked immunosorbent assay.

RESULTS:

We found that medulloblastoma growth in IL-17-injected mice was significantly inhibited compared to the non-IL-17 treated mice. In contrast to the IL-17 antitumor activity observed in mice injected with splenocytes, we observed that IFN-gamma, IL-6, IL-23, Ccl2, and Ccl20 proteins were significantly increased in tumor tissues of mice injected with IL-17.

CONCLUSIONS:

These experiments suggest that IL-17 may promote splenocyte antitumor activity in medulloblastoma. We postulate that IL-17's antitumor activity may be related to the increased protein levels of IFN-gamma, IL-6, IL-23, Ccl2, and Ccl20.

• Authors: Ping Zhou, Qilin Zhang, Yao Zhao, Hongying Sha, Xiaoyun Cao, Yongfei Wang
• Journal: European journal of medical research (Eur J Med Res) Vol. 20 Pg. 98 (Dec 18 2015) ISSN: 2047-783X [Electronic] England
• PMID: 26684834 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• CCL20 protein, mouse
• Ccl2 protein, mouse
• Chemokine CCL2
• Chemokine CCL20
• Interleukin-17
• Interleukin-23
• Interleukin-6
• Interferon-gamma

Topics

• Animals
• Antineoplastic Agents (administration & dosage, pharmacology)
• Cerebellar Neoplasms (drug therapy, genetics, pathology)
• Chemokine CCL2 (metabolism)
• Chemokine CCL20 (metabolism)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Injections
• Interferon-gamma (metabolism)
• Interleukin-17 (administration & dosage, metabolism, pharmacology)
• Interleukin-23 (genetics)
• Interleukin-6 (genetics)
• Medulloblastoma (drug therapy, genetics, pathology)
• Mice, Inbred BALB C
• Mice, Nude
• Spleen (cytology)
• Tumor Cells, Cultured
• Xenograft Model Antitumor Assays