Acute liver injury is a debilitating disorder associated with loss of synthetic and detoxifying functions of the liver. This investigation was designed to assess cytoprotective efficacy of daily oral
tiron (300 mg/kg) and daily oral
methyl palmitate (300 mg/kg) against
acetaminophen-induced acute liver injury. Rats were orally pretreated with either
tiron or
methyl palmitate at doses (300 mg/kg) for 7 days prior to oral
acetaminophen (3 g/kg). Biochemical assay of markers of hepatotoxicity indices and oxidative stress was undertaken. Expression of inflammatory
cytokine IL-6 was also evaluated. Histopathological examination of liver specimens was carried out as well. Both
methyl palmitate and
tiron significantly reversed the
acetaminophen-induced elevation of
biochemical markers (ALT, AST, and ALP) with restoration of SOD levels.
Serum albumin levels and GSH liver contents increased, but in a nonsignificant manner. Moreover,
methyl palmitate and
tiron significantly decreased the level of serum LDH and serum
IL-6 levels. Histopathology revealed that
tiron markedly reduced the extent of
acetaminophen-induced
necrosis and
methyl palmitate moderately decreased the
necrosis in liver tissue.
Methyl palmitate (300 mg/kg) and
tiron (300 mg/kg) demonstrated promising hepatoprotective effects against
acetaminophen-induced acute liver injury via modulation of inflammatory response and alleviation of the oxidative stress, allowing the preservation of hepatic functions.