To investigate
vitamin D-related control of brain-expressed genes, candidate
vitamin D responsive elements (VDREs) at -7/-10 kb in human
tryptophan hydroxylase (TPH)2 were probed. Both VDREs bound the
vitamin D receptor (VDR)-
retinoid X receptor (RXR) complex and drove reporter gene transcription in response to
1,25-dihydroxyvitamin D3 (1,25D). Brain TPH2
mRNA, encoding the rate-limiting
enzyme in
serotonin synthesis, was induced 2.2-fold by 10 nM 1,25D in human U87
glioblastoma cells and 47.8-fold in rat serotonergic RN46A-B14 cells. 1,25D regulation of
leptin (Lep), encoding a serotoninlike satiety factor, was also examined. In mouse adipocytes, 1,25D repressed
leptin mRNA levels by at least 84%, whereas 1,25D induced
leptin mRNA 15.1-fold in human
glioblastoma cells.
Chromatin immunoprecipitation sequencing analysis of the mouse Lep gene in response to 1,25D revealed a cluster of regulatory sites (cis-regulatory module; CRM) at -28 kb that 1,25D-dependently docked VDR, RXR, C/EBPβ, and RUNX2. This CRM harbored 3 VDREs and single C/EBPβ and RUNX2 sites. Therefore, the expression of human TPH2 and mouse Lep are governed by 1,25D, potentially via respective VDREs located at -7/-10 kb and -28 kb. These results imply that
vitamin D affects brain
serotonin concentrations, which may be relevant to
psychiatric disorders, such as
autism, and may control
leptin levels and affect eating behavior.