Abstract | BACKGROUND: RESULTS: A total of five MMP genes were identified in tomato genome. qRT-PCR analysis revealed that expression of Sl- MMP genes was induced with distinct patterns by infection of Botrytis cinerea and Pseudomonas syringae pv. tomato (Pst) DC3000 and by treatment with defense-related hormones such as salicylic acid, jasmonic acid and ethylene precursor 1-amino cyclopropane-1-carboxylic acid. Virus-induced gene silencing (VIGS)-based knockdown of individual Sl- MMPs and disease assays indicated that silencing of Sl3-MMP resulted in reduced resistance to B. cinerea and Pst DC3000, whereas silencing of other four Sl- MMPs did not affect the disease resistance against these two pathogens. The Sl3-MMP-silenced tomato plants responded with increased accumulation of reactive oxygen species and alerted expression of defense genes after infection of B. cinerea. Transient expression of Sl3-MMP in leaves of Nicotiana benthamiana led to an enhanced resistance to B. cinerea and upregulated expression of defense-related genes. Biochemical assays revealed that the recombinant mature Sl3-MMP protein had proteolytic activities in vitro with distinct preferences for specificity of cleavage sites. The Sl3-MMP protein was targeted onto the plasma membrane of plant cells when transiently expressed in onion epidermal cells. CONCLUSION: VIGS-based knockdown of Sl3-MMP expression in tomato and gain-of-function transient expression of Sl3-MMP in N. benthamiana demonstrate that Sl3-MMP functions as a positive regulator of defense response against B. cinerea and Pst DC3000.
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Authors | Dayong Li, Huijuan Zhang, Qiuming Song, Lu Wang, Shixia Liu, Yongbo Hong, Lei Huang, Fengming Song |
Journal | BMC plant biology
(BMC Plant Biol)
Vol. 15
Pg. 143
(Jun 14 2015)
ISSN: 1471-2229 [Electronic] England |
PMID | 26070456
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Growth Regulators
- Plant Proteins
- Hydrogen Peroxide
- Matrix Metalloproteinases
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Topics |
- Amino Acid Sequence
- Botrytis
(drug effects, physiology)
- Cell Death
(drug effects)
- Cell Membrane
(drug effects, metabolism)
- Disease Resistance
(drug effects, immunology)
- Gene Expression Regulation, Plant
(drug effects)
- Gene Silencing
(drug effects)
- Genes, Plant
- Hydrogen Peroxide
(metabolism)
- Solanum lycopersicum
(enzymology, genetics, immunology, microbiology)
- Matrix Metalloproteinases
(chemistry, metabolism)
- Molecular Sequence Data
- Multigene Family
- Onions
(cytology)
- Phylogeny
- Plant Diseases
(genetics, immunology, microbiology)
- Plant Epidermis
(cytology, drug effects, metabolism)
- Plant Growth Regulators
(pharmacology)
- Plant Proteins
(chemistry, metabolism)
- Plants, Genetically Modified
- Protein Transport
(drug effects)
- Proteolysis
(drug effects)
- Pseudomonas syringae
(drug effects, physiology)
- Sequence Alignment
- Signal Transduction
(drug effects, genetics)
- Subcellular Fractions
(drug effects, metabolism)
- Nicotiana
(drug effects, genetics)
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