Abstract | BACKGROUND: METHODS: We measured cholesterol efflux capacity and assessed its relation with vascular risk factors and incident coronary heart disease events in a nested case-control sample from the prospective EPIC-Norfolk study of 25 639 individuals aged 40-79 years, assessed in 1993-97 and followed up to 2009. We quantified cholesterol efflux capacity in 1745 patients with incident coronary heart disease and 1749 control participants free of any cardiovascular disorders by use of a validated ex-vivo radiotracer assay that involved incubation of cholesterol-labelled J774 macrophages with apoB-depleted serum from study participants. FINDINGS:
Cholesterol efflux capacity was positively correlated with HDL cholesterol concentration (r=0·40; p<0·0001) and apoA-I concentration (r=0·22; p<0·0001). It was also inversely correlated with type 2 diabetes (r=-0·18; p<0·0001) and positively correlated with alcohol consumption (r=0·12; p<0·0001). In analyses comparing the top and bottom tertiles, cholesterol efflux capacity was significantly and inversely associated with incident coronary heart disease events, independent of age, sex, diabetes, hypertension, smoking and alcohol use, waist:hip ratio, BMI, LDL cholesterol concentration, log- triglycerides, and HDL cholesterol or apoA-I concentrations (odds ratio 0·64, 95% CI 0·51-0·80). After a similar multivariable adjustment the risk of incident coronary heart disease was 0·80 (95% CI 0·70-0·90) for a per-SD change in cholesterol efflux capacity. INTERPRETATION: FUNDING: US National Institutes of Health, UK Medical Research Council, Cancer Research UK.
|
Authors | Danish Saleheen, Robert Scott, Sundas Javad, Wei Zhao, Amrith Rodrigues, Antonino Picataggi, Daniya Lukmanova, Megan L Mucksavage, Robert Luben, Jeffery Billheimer, John J P Kastelein, S Matthijs Boekholdt, Kay-Tee Khaw, Nick Wareham, Daniel J Rader |
Journal | The lancet. Diabetes & endocrinology
(Lancet Diabetes Endocrinol)
Vol. 3
Issue 7
Pg. 507-13
(Jul 2015)
ISSN: 2213-8595 [Electronic] England |
PMID | 26025389
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2015 Saleheen et al. Open Acess article disrtibuted under the terms of CC BY-NC-ND. Published by Elsevier Ltd.. All rights reserved. |
Chemical References |
|
Topics |
- Adult
- Aged
- Case-Control Studies
- Cholesterol, HDL
(blood)
- Coronary Disease
(blood, epidemiology)
- Female
- Humans
- Male
- Middle Aged
- Prospective Studies
- Risk Factors
|